01270naa a2200229 a 450000100080000000500110000800800410001910000160006024501130007626000090018952006300019865000110082865000120083965300130085165300130086465300120087765300250088965300260091465300150094065300170095577300680097216479892023-05-17       1993    bl uuuu     u00u1 u    #d1 aNiedz, R. P  aCulturing embryogenic protoplasts of 'Hamlin' sweet orange in calcium alginate beads.h[electronic resource]  c1993  aProtoplasts were enzymatically isolated from log embryogenic sweet orang [Citrus sinensis (L.) Osbeck cv. Hamlin] suspension cultures embedded in 3-mm diameter Ca-alginate beads, and cultured in a growth cabinet (15-20u mol m-2 s-1, 4-h photoperiod, 27oC). Plating efficiency exceeded 90% for Ca-alginate embedded protoplasts vs. 30% for protoplasts cultured in a liquid medium. Embryoids formed from protoplasts were recoverd after 20 days by dissolving the Ca-alginate matrix with a calcium sequestrant. embryoids readily formed shoots that were rooted on MS + 0.0luM NAA + 5% sucrose. Potential applications are discussed.  aCitrus  aCálcio  aAlginate  aAlginato  aCalciun  aEmbriogenese Somatic  aEmbriogenese Somatica  aProtoplast  aProtoplastos  tPlant Cell, Tissue and Organ Culturegv.34, n.1, p.19-25, 1993.