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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
11/12/2000 |
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Data da última atualização: |
04/08/2023 |
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Autoria: |
ORDEN, J. A.; GOYACHE, J.; HERNANDEZ, J.; DOMENECH, A.; SUAREZ, G.; GOMEZ-LUCIA, E. |
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Título: |
Detection of enterotoxins and TSST-1 secreted by Staphylococcus aureus isolated from ruminant mastitis. Comparison of ELISA and immunoblot. |
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Ano de publicação: |
1992 |
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Fonte/Imprenta: |
The Journal of Applied Microbiology, v. 72, n. 6, p. 486-489, Jun. 1992. |
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DOI: |
10.1111/j.1365-2672.1992.tb01863.x. |
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Idioma: |
Inglês |
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Conteúdo: |
The production of staphylococcal enterotoxins (SE) and toxic shock syndrome toxin-1 (TSST-1) was studied in 81 strains of Staphylococcus aureus isolated from cases of mastitis in cattle, goats and sheep. SE and TSST-1 were detected by two techniques: ELISA double antibody sandwich, and an immunoblot technique combined with a semiautomated electrophoresis system. More Staph. aureus strains isolated from sheep produced enterotoxins than those from goats and cattle. SEC was the predominant type in all isolates from these animal species. The highest proportion of strains producing TSST-1 were obtained from sheep, twice as many as those from goats or cows. The two techniques gave similar results. as all the strains positive by immunoblot were also positive by ELISA, and only three were positive by ELISA but negative by immunoblot. |
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Palavras-Chave: |
Mastite; Staphylococcys aureus. |
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Thesagro: |
Staphylococcus Aureus. |
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Thesaurus Nal: |
Bacterial toxins; Biosynthesis; Bovine mastitis; Cattle diseases; Enterotoxins; Enzyme-linked immunosorbent assay; Goat diseases; Immunoblotting; Mastitis; Microbiology; Sheep diseases; Staphylococcal intoxication; Superantigens. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02022naa a2200385 a 4500
001 1524193
005 2023-08-04
008 1992 bl uuuu u00u1 u #d
024 7 $a10.1111/j.1365-2672.1992.tb01863.x.$2DOI
100 1 $aORDEN, J. A.
245 $aDetection of enterotoxins and TSST-1 secreted by Staphylococcus aureus isolated from ruminant mastitis. Comparison of ELISA and immunoblot.$h[electronic resource]
260 $c1992
520 $aThe production of staphylococcal enterotoxins (SE) and toxic shock syndrome toxin-1 (TSST-1) was studied in 81 strains of Staphylococcus aureus isolated from cases of mastitis in cattle, goats and sheep. SE and TSST-1 were detected by two techniques: ELISA double antibody sandwich, and an immunoblot technique combined with a semiautomated electrophoresis system. More Staph. aureus strains isolated from sheep produced enterotoxins than those from goats and cattle. SEC was the predominant type in all isolates from these animal species. The highest proportion of strains producing TSST-1 were obtained from sheep, twice as many as those from goats or cows. The two techniques gave similar results. as all the strains positive by immunoblot were also positive by ELISA, and only three were positive by ELISA but negative by immunoblot.
650 $aBacterial toxins
650 $aBiosynthesis
650 $aBovine mastitis
650 $aCattle diseases
650 $aEnterotoxins
650 $aEnzyme-linked immunosorbent assay
650 $aGoat diseases
650 $aImmunoblotting
650 $aMastitis
650 $aMicrobiology
650 $aSheep diseases
650 $aStaphylococcal intoxication
650 $aSuperantigens
650 $aStaphylococcus Aureus
653 $aMastite
653 $aStaphylococcys aureus
700 1 $aGOYACHE, J.
700 1 $aHERNANDEZ, J.
700 1 $aDOMENECH, A.
700 1 $aSUAREZ, G.
700 1 $aGOMEZ-LUCIA, E.
773 $tThe Journal of Applied Microbiology$gv. 72, n. 6, p. 486-489, Jun. 1992.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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