Registro Completo |
Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
Data corrente: |
11/12/2000 |
Data da última atualização: |
04/08/2023 |
Autoria: |
ORDEN, J. A.; GOYACHE, J.; HERNANDEZ, J.; DOMENECH, A.; SUAREZ, G.; GOMEZ-LUCIA, E. |
Título: |
Detection of enterotoxins and TSST-1 secreted by Staphylococcus aureus isolated from ruminant mastitis. Comparison of ELISA and immunoblot. |
Ano de publicação: |
1992 |
Fonte/Imprenta: |
The Journal of Applied Microbiology, v. 72, n. 6, p. 486-489, Jun. 1992. |
DOI: |
10.1111/j.1365-2672.1992.tb01863.x. |
Idioma: |
Inglês |
Conteúdo: |
The production of staphylococcal enterotoxins (SE) and toxic shock syndrome toxin-1 (TSST-1) was studied in 81 strains of Staphylococcus aureus isolated from cases of mastitis in cattle, goats and sheep. SE and TSST-1 were detected by two techniques: ELISA double antibody sandwich, and an immunoblot technique combined with a semiautomated electrophoresis system. More Staph. aureus strains isolated from sheep produced enterotoxins than those from goats and cattle. SEC was the predominant type in all isolates from these animal species. The highest proportion of strains producing TSST-1 were obtained from sheep, twice as many as those from goats or cows. The two techniques gave similar results. as all the strains positive by immunoblot were also positive by ELISA, and only three were positive by ELISA but negative by immunoblot. |
Palavras-Chave: |
Mastite; Staphylococcys aureus. |
Thesagro: |
Staphylococcus Aureus. |
Thesaurus Nal: |
Bacterial toxins; Biosynthesis; Bovine mastitis; Cattle diseases; Enterotoxins; Enzyme-linked immunosorbent assay; Goat diseases; Immunoblotting; Mastitis; Microbiology; Sheep diseases; Staphylococcal intoxication; Superantigens. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02022naa a2200385 a 4500 001 1524193 005 2023-08-04 008 1992 bl uuuu u00u1 u #d 024 7 $a10.1111/j.1365-2672.1992.tb01863.x.$2DOI 100 1 $aORDEN, J. A. 245 $aDetection of enterotoxins and TSST-1 secreted by Staphylococcus aureus isolated from ruminant mastitis. Comparison of ELISA and immunoblot.$h[electronic resource] 260 $c1992 520 $aThe production of staphylococcal enterotoxins (SE) and toxic shock syndrome toxin-1 (TSST-1) was studied in 81 strains of Staphylococcus aureus isolated from cases of mastitis in cattle, goats and sheep. SE and TSST-1 were detected by two techniques: ELISA double antibody sandwich, and an immunoblot technique combined with a semiautomated electrophoresis system. More Staph. aureus strains isolated from sheep produced enterotoxins than those from goats and cattle. SEC was the predominant type in all isolates from these animal species. The highest proportion of strains producing TSST-1 were obtained from sheep, twice as many as those from goats or cows. The two techniques gave similar results. as all the strains positive by immunoblot were also positive by ELISA, and only three were positive by ELISA but negative by immunoblot. 650 $aBacterial toxins 650 $aBiosynthesis 650 $aBovine mastitis 650 $aCattle diseases 650 $aEnterotoxins 650 $aEnzyme-linked immunosorbent assay 650 $aGoat diseases 650 $aImmunoblotting 650 $aMastitis 650 $aMicrobiology 650 $aSheep diseases 650 $aStaphylococcal intoxication 650 $aSuperantigens 650 $aStaphylococcus Aureus 653 $aMastite 653 $aStaphylococcys aureus 700 1 $aGOYACHE, J. 700 1 $aHERNANDEZ, J. 700 1 $aDOMENECH, A. 700 1 $aSUAREZ, G. 700 1 $aGOMEZ-LUCIA, E. 773 $tThe Journal of Applied Microbiology$gv. 72, n. 6, p. 486-489, Jun. 1992.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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