02022naa a2200385 a 450000100080000000500110000800800410001902400450006010000170010524501670012226000090028952008420029865000210114065000170116165000200117865000200119865000170121865000380123565000180127365000190129165000130131065000170132365000190134065000320135965000180139165000260140965300120143565300260144770000160147370000180148970000170150770000150152470000200153977300770155915241932023-08-04       1992    bl uuuu     u00u1 u    #d7 a10.1111/j.1365-2672.1992.tb01863.x.2DOI1 aORDEN, J. A.  aDetection of enterotoxins and TSST-1 secreted by Staphylococcus aureus isolated from ruminant mastitis. Comparison of ELISA and immunoblot.h[electronic resource]  c1992  aThe production of staphylococcal enterotoxins (SE) and toxic shock syndrome toxin-1 (TSST-1) was studied in 81 strains of Staphylococcus aureus isolated from cases of mastitis in cattle, goats and sheep. SE and TSST-1 were detected by two techniques: ELISA double antibody sandwich, and an immunoblot technique combined with a semiautomated electrophoresis system. More Staph. aureus strains isolated from sheep produced enterotoxins than those from goats and cattle. SEC was the predominant type in all isolates from these animal species. The highest proportion of strains producing TSST-1 were obtained from sheep, twice as many as those from goats or cows. The two techniques gave similar results. as all the strains positive by immunoblot were also positive by ELISA, and only three were positive by ELISA but negative by immunoblot.  aBacterial toxins  aBiosynthesis  aBovine mastitis  aCattle diseases  aEnterotoxins  aEnzyme-linked immunosorbent assay  aGoat diseases  aImmunoblotting  aMastitis  aMicrobiology  aSheep diseases  aStaphylococcal intoxication  aSuperantigens  aStaphylococcus Aureus  aMastite  aStaphylococcys aureus1 aGOYACHE, J.1 aHERNANDEZ, J.1 aDOMENECH, A.1 aSUAREZ, G.1 aGOMEZ-LUCIA, E.  tThe Journal of Applied Microbiologygv. 72, n. 6, p. 486-489, Jun. 1992.