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 | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
27/09/1996 |
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Data da última atualização: |
13/06/2023 |
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Autoria: |
NATH, J.; PATT JUNIOR, J. |
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Título: |
Biochemical changes associated with freezing in ram semen. |
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Ano de publicação: |
1970 |
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Fonte/Imprenta: |
Cryobiology, v. 6, n. 6, p. 522-528, May/Jun. 1970. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: The effects of freezing and thawing upon ovine spermatozoal acid phosphatase, lactate dehydrogenase, and deoxyribonucleic acid were determined. Two bands of seminal acid phosphatase (P-AP1 and P-AP2) and two bands of spermatozoal acid phosphatase (S-AP1 and S-AP2) were detected by acrylamide gel electrophoresis. Seven bands of both seminal and spermatozoal lactate dehydrogenase activity were also observed. Freezing and thawing produced a significant increase in the activity of P-AP2 band of seminal plasma and a concomitant significant decrease in the activity of S-AP2 band in spermatozoa (P < 0.05). This suggests that some S-AP2 isozyme leaks out of the sperm into the diluent. The three lactate dehydrogenase isozymes measured did not show alterations in activity following freezing, thawing, or incubation. The deoxyribonucleic acid content of ram spermatozoa remained unaffected by freezing, thawing, or incubation. |
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Palavras-Chave: |
Congelacao; Isoenzymes; L-Lactate Dehydrogenase. |
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Thesagro: |
Bioquímica; DNA; Ovino; Sêmen. |
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Thesaurus Nal: |
Acid phosphatase; Densitometry; Metabolism; Sheep; Spermatozoa. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 01647naa a2200277 a 4500
001 1523284
005 2023-06-13
008 1970 bl uuuu u00u1 u #d
100 1 $aNATH, J.
245 $aBiochemical changes associated with freezing in ram semen.$h[electronic resource]
260 $c1970
520 $aAbstract: The effects of freezing and thawing upon ovine spermatozoal acid phosphatase, lactate dehydrogenase, and deoxyribonucleic acid were determined. Two bands of seminal acid phosphatase (P-AP1 and P-AP2) and two bands of spermatozoal acid phosphatase (S-AP1 and S-AP2) were detected by acrylamide gel electrophoresis. Seven bands of both seminal and spermatozoal lactate dehydrogenase activity were also observed. Freezing and thawing produced a significant increase in the activity of P-AP2 band of seminal plasma and a concomitant significant decrease in the activity of S-AP2 band in spermatozoa (P < 0.05). This suggests that some S-AP2 isozyme leaks out of the sperm into the diluent. The three lactate dehydrogenase isozymes measured did not show alterations in activity following freezing, thawing, or incubation. The deoxyribonucleic acid content of ram spermatozoa remained unaffected by freezing, thawing, or incubation.
650 $aAcid phosphatase
650 $aDensitometry
650 $aMetabolism
650 $aSheep
650 $aSpermatozoa
650 $aBioquímica
650 $aDNA
650 $aOvino
650 $aSêmen
653 $aCongelacao
653 $aIsoenzymes
653 $aL-Lactate Dehydrogenase
700 1 $aPATT JUNIOR, J.
773 $tCryobiology$gv. 6, n. 6, p. 522-528, May/Jun. 1970.
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| 1. |  | AGUIAR NETO, A. de O.; GOMES, C. C. S.; LINS, C. C. V.; BARROS, A. C.; CAMPECHE, L. F. de S. M.; BLANCO, F. F. Características químicas e salino-sodicidade dos solos do Perímetro Irrigado Califórnia, SE, Brasil. Ciência Rural, Santa Maria, RS, v. 37, n. 6, p. 1640-1645, nov./dez. 2007.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Nacional - A |
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