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 | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
26/03/1996 |
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Data da última atualização: |
05/12/2023 |
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Autoria: |
McNAMARA, P. J.; BRADLY, G. A.; SONGER, J. G. |
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Título: |
Targeted mutagenesis of the phospholipase d gene results in decreased virulence of Corynebacterium pseudotuberculosis. |
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Ano de publicação: |
1994 |
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Fonte/Imprenta: |
Molecular Microbiologia, v. 12, n. 6, p. 921-930, Jun. 1994. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: The chromosomal gene encoding the phospholipase D from Corynebacterium pseudotuberculosis (biovar ovis) isolate Whetten 1 was replaced with an allele containing a nonsense mutation. The virulence of the mutant strain (W1.31r1) and the isogenic parental strain were then compared by inoculation of goats. The wild-type strain caused abscessation at the site of infection, which then spread to the regional lymph node, while W1.31r1 had a reduced ability to establish a primary infection and was incapable of dissemination. Our results confirm that phospholipase D is a virulence determinant of C. pseudotuberculosis that increases the persistence and spread of the bacteria within the host. |
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Palavras-Chave: |
Corybacterium pseudotuberculosis; Microbiologia molecular. |
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Thesagro: |
Corynebacterium Pseudotuberculosis; Linfadenite Caseosa. |
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Thesaurus Nal: |
Amino acid sequences; Enzymology; Gene targeting; Goat diseases; Goats; Microbiology; Mutagenesis; Mutation; Phospholipase D; Physiology; Virulence. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01676naa a2200325 a 4500
001 1522010
005 2023-12-05
008 1994 bl uuuu u00u1 u #d
100 1 $aMcNAMARA, P. J.
245 $aTargeted mutagenesis of the phospholipase d gene results in decreased virulence of Corynebacterium pseudotuberculosis.$h[electronic resource]
260 $c1994
520 $aAbstract: The chromosomal gene encoding the phospholipase D from Corynebacterium pseudotuberculosis (biovar ovis) isolate Whetten 1 was replaced with an allele containing a nonsense mutation. The virulence of the mutant strain (W1.31r1) and the isogenic parental strain were then compared by inoculation of goats. The wild-type strain caused abscessation at the site of infection, which then spread to the regional lymph node, while W1.31r1 had a reduced ability to establish a primary infection and was incapable of dissemination. Our results confirm that phospholipase D is a virulence determinant of C. pseudotuberculosis that increases the persistence and spread of the bacteria within the host.
650 $aAmino acid sequences
650 $aEnzymology
650 $aGene targeting
650 $aGoat diseases
650 $aGoats
650 $aMicrobiology
650 $aMutagenesis
650 $aMutation
650 $aPhospholipase D
650 $aPhysiology
650 $aVirulence
650 $aCorynebacterium Pseudotuberculosis
650 $aLinfadenite Caseosa
653 $aCorybacterium pseudotuberculosis
653 $aMicrobiologia molecular
700 1 $aBRADLY, G. A.
700 1 $aSONGER, J. G.
773 $tMolecular Microbiologia$gv. 12, n. 6, p. 921-930, Jun. 1994.
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Embrapa Caprinos e Ovinos (CNPC) |
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| 2. |  | OJEDA FILHO, S. C. F.; RIBEIRO, L. J.; MESSIAS, E. A. C.; BARROS, B. V.; AHMED CHEIKH, N.; BARROS, L. P.; SANCHES, V. J. S.; SOUZA LIMA, E. S.; VARELA, L. H. A.; TUCAN, E. de O. AVELLAR, W.; SILVA, R. A. M. S. Parasita gastrointestinais de equinos do Pantanal mantidos em Cluibe do Laco. In: CONGRESSO DE INICIACAO CIENTIFICA EM CIENCIAS AGRARIAS, BIOLOGICAS E AMBIENTAIS, 1., 2003, Sao Paulo. Resumos: Arq. Inst. Biol., Sao Paulo [CD-ROM], v.70, supl. 2, 2003. Resumo 5AC.| Biblioteca(s): Embrapa Pantanal. |
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