01676naa a2200325 a 450000100080000000500110000800800410001910000200006024501460008026000090022652007040023565000250093965000150096465000190097965000180099865000100101665000170102665000160104365000130105965000200107265000150109265000140110765000390112165000240116065300370118465300280122170000180124970000180126777300650128515220102023-12-05       1994    bl uuuu     u00u1 u    #d1 aMcNAMARA, P. J.  aTargeted mutagenesis of the phospholipase d gene results in decreased virulence of Corynebacterium pseudotuberculosis.h[electronic resource]  c1994  aAbstract: The chromosomal gene encoding the phospholipase D from Corynebacterium pseudotuberculosis (biovar ovis) isolate Whetten 1 was replaced with an allele containing a nonsense mutation. The virulence of the mutant strain (W1.31r1) and the isogenic parental strain were then compared by inoculation of goats. The wild-type strain caused abscessation at the site of infection, which then spread to the regional lymph node, while W1.31r1 had a reduced ability to establish a primary infection and was incapable of dissemination. Our results confirm that phospholipase D is a virulence determinant of C. pseudotuberculosis that increases the persistence and spread of the bacteria within the host.  aAmino acid sequences  aEnzymology  aGene targeting  aGoat diseases  aGoats  aMicrobiology  aMutagenesis  aMutation  aPhospholipase D  aPhysiology  aVirulence  aCorynebacterium Pseudotuberculosis  aLinfadenite Caseosa  aCorybacterium pseudotuberculosis  aMicrobiologia molecular1 aBRADLY, G. A.1 aSONGER, J. G.  tMolecular Microbiologiagv. 12, n. 6, p. 921-930, Jun. 1994.