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 | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
01/08/1992 |
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Data da última atualização: |
08/01/2024 |
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Autoria: |
O'DONNELL, I. J.; DINEEN, J. K.; ROTHWELL. T. L. W.; MARSHALL, R. C. |
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Título: |
Attempts to probe the antigens and protective immunogens of trichostrongylus colubriformis in immunoblots with sera from infected and hyperimmune sheep and high and low-responder guinea pigs. |
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Ano de publicação: |
1985 |
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Fonte/Imprenta: |
International Journal Parasitology, v. 15, p. 129-136, 1985. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Comparison of antisera from sheep during primary infection and following vaccination and challenge with Trichostrongylus colubriformis, with antisera obtained following primary infection of high- and low-responder guinea pigs, failed to reveal different antigenic patterns in proteins separated from fourth stage larval extracts by two-dimensional electrophoresis and probed by the immunoblot technique. Generally, serum IgG reacted specifically with worm antigens of mol. wt greater than 94,000, whereas protection against challenge infection was elicited most effectively in the guinea pig by fractions in the 67,000?94,000 range. Most distinct separations of larval proteins by SDS-polyacrylamide gel electrophoresis were obtained by extraction of live larvae and the extracts used within 2?3 days. |
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Palavras-Chave: |
Immunoblots; Nematodeo; Protective immunogens. |
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Thesaurus Nal: |
Antigens; Guinea pigs; Nematoda; Parasitology; Sheep; Trichostrongylus colubriformis. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 01677naa a2200265 a 4500
001 1521786
005 2024-01-08
008 1985 bl uuuu u00u1 u #d
100 1 $aO'DONNELL, I. J.
245 $aAttempts to probe the antigens and protective immunogens of trichostrongylus colubriformis in immunoblots with sera from infected and hyperimmune sheep and high and low-responder guinea pigs.$h[electronic resource]
260 $c1985
520 $aAbstract: Comparison of antisera from sheep during primary infection and following vaccination and challenge with Trichostrongylus colubriformis, with antisera obtained following primary infection of high- and low-responder guinea pigs, failed to reveal different antigenic patterns in proteins separated from fourth stage larval extracts by two-dimensional electrophoresis and probed by the immunoblot technique. Generally, serum IgG reacted specifically with worm antigens of mol. wt greater than 94,000, whereas protection against challenge infection was elicited most effectively in the guinea pig by fractions in the 67,000?94,000 range. Most distinct separations of larval proteins by SDS-polyacrylamide gel electrophoresis were obtained by extraction of live larvae and the extracts used within 2?3 days.
650 $aAntigens
650 $aGuinea pigs
650 $aNematoda
650 $aParasitology
650 $aSheep
650 $aTrichostrongylus colubriformis
653 $aImmunoblots
653 $aNematodeo
653 $aProtective immunogens
700 1 $aDINEEN, J. K.
700 1 $aROTHWELL. T. L. W.
700 1 $aMARSHALL, R. C.
773 $tInternational Journal Parasitology$gv. 15, p. 129-136, 1985.
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Embrapa Caprinos e Ovinos (CNPC) |
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| 1. |  | COELHO, I. L.; LARANJEIRA, D.; FERRAZ, L. G. B.; ASSIS, T. C. D.; CARVALHO, R. R. D. C. E.; BOARI, A. D. J.; SANTANA, F. C. T. D. Potencialidade de levedura como biocontrolador in vitro de Lasiodiplodia teobromae. In: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 46.; REUNIÃO BRASILEIRA DE CONTROLE BIOLÓGICO, 11., 2013, Ouro Preto. CBfito sustentável. Ouro Preto: UFV, 2013. 1 CD-ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 2. | | COELHO, I. L.; LARANJEIRA, D.; FERRAZ, L. G. B.; ASSIS, T. C. D.; CARVALHO, R. R. D. C. E.; TREMACOLDI, C. R. Métodos de inoculação de Thielaviopsis paradoxa em mudas de coqueiro-anão-verde. In: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 46.; REUNIÃO BRASILEIRA DE CONTROLE BIOLÓGICO, 11., 2013, Ouro Preto. CBfito sustentável. Ouro Preto: UFV, 2013. 1 CD-ROM. Resumo 567-1.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| Registros recuperados : 2 | |
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