01677naa a2200265 a 450000100080000000500110000800800410001910000210006024502190008126000090030052008160030965000130112565000160113865000130115465000170116765000100118465000350119465300160122965300140124565300260125970000180128570000230130370000200132677300650134615217862024-01-08       1985    bl uuuu     u00u1 u    #d1 aO'DONNELL, I. J.  aAttempts to probe the antigens and protective immunogens of trichostrongylus colubriformis in immunoblots with sera from infected and hyperimmune sheep and high and low-responder guinea pigs.h[electronic resource]  c1985  aAbstract: Comparison of antisera from sheep during primary infection and following vaccination and challenge with Trichostrongylus colubriformis, with antisera obtained following primary infection of high- and low-responder guinea pigs, failed to reveal different antigenic patterns in proteins separated from fourth stage larval extracts by two-dimensional electrophoresis and probed by the immunoblot technique. Generally, serum IgG reacted specifically with worm antigens of mol. wt greater than 94,000, whereas protection against challenge infection was elicited most effectively in the guinea pig by fractions in the 67,000?94,000 range. Most distinct separations of larval proteins by SDS-polyacrylamide gel electrophoresis were obtained by extraction of live larvae and the extracts used within 2?3 days.  aAntigens  aGuinea pigs  aNematoda  aParasitology  aSheep  aTrichostrongylus colubriformis  aImmunoblots  aNematodeo  aProtective immunogens1 aDINEEN, J. K.1 aROTHWELL. T. L. W.1 aMARSHALL, R. C.  tInternational Journal Parasitologygv. 15, p. 129-136, 1985.