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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
01/08/1992 |
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Data da última atualização: |
02/08/2023 |
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Autoria: |
LIGHTFOOT, R. J.; SALAMON, S. |
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Título: |
Freezing ram spermatozoa by the pellet method. II. The effects of method of dilution, dilution rate, glycerol concentration, and duration of storage at 5oC prior to freezing on survival of spermatozoa. |
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Ano de publicação: |
1960 |
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Fonte/Imprenta: |
Australian Journal of Biological Sciences, v. 22, n. 6, p. 547-560, 1960. |
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DOI: |
10.1071/BI9691547 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Five factorial experiments were conducted to examine the relationship between the effects of diluent composition, method of dilution, dilution rate, glycerol con-centration, duration of storage at 5°C prior to freezing, and dilution rate at thawing on the survival of ram spermatozoa after freezing by the pellet method. The optimal method of dilution, glycerol concentration, and duration of storage were found to vary according to the composition of the diluent. With moderately hypertonic (6. = O· 7-0'9 degC) raffinose-yolk-citrate diluents, best post-thawing survival of spermatozoa was achieved by dilution with the glycerol-containing diluent at 30°C prior to cooling, with 3-4% glycerol in the diluted semen, and by a short (1 hr) period of storage at 5°C prior to pellet freezing. |
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Palavras-Chave: |
Diluidor; Semen diluents; Semen preservation; Tecnologia do sêmen. |
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Thesagro: |
Congelamento; Espermatozóide; Ovino; Reprodução; Sêmen; Sobrevivência. |
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Thesaurus Nal: |
Embryo transfer; Reproduction; Sheep; Spermatozoa. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 01799naa a2200313 a 4500
001 1521549
005 2023-08-02
008 1960 bl uuuu u00u1 u #d
024 7 $a10.1071/BI9691547$2DOI
100 1 $aLIGHTFOOT, R. J.
245 $aFreezing ram spermatozoa by the pellet method. II. The effects of method of dilution, dilution rate, glycerol concentration, and duration of storage at 5oC prior to freezing on survival of spermatozoa.$h[electronic resource]
260 $c1960
520 $aAbstract: Five factorial experiments were conducted to examine the relationship between the effects of diluent composition, method of dilution, dilution rate, glycerol con-centration, duration of storage at 5°C prior to freezing, and dilution rate at thawing on the survival of ram spermatozoa after freezing by the pellet method. The optimal method of dilution, glycerol concentration, and duration of storage were found to vary according to the composition of the diluent. With moderately hypertonic (6. = O· 7-0'9 degC) raffinose-yolk-citrate diluents, best post-thawing survival of spermatozoa was achieved by dilution with the glycerol-containing diluent at 30°C prior to cooling, with 3-4% glycerol in the diluted semen, and by a short (1 hr) period of storage at 5°C prior to pellet freezing.
650 $aEmbryo transfer
650 $aReproduction
650 $aSheep
650 $aSpermatozoa
650 $aCongelamento
650 $aEspermatozóide
650 $aOvino
650 $aReprodução
650 $aSêmen
650 $aSobrevivência
653 $aDiluidor
653 $aSemen diluents
653 $aSemen preservation
653 $aTecnologia do sêmen
700 1 $aSALAMON, S.
773 $tAustralian Journal of Biological Sciences$gv. 22, n. 6, p. 547-560, 1960.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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