01799naa a2200313 a 450000100080000000500110000800800410001902400270006010000210008724502290010826000090033752008080034665000200115465000170117465000100119165000160120165000170121765000200123465000100125465000170126465000110128165000190129265300130131165300190132465300230134365300250136670000160139177300780140715215492023-08-02       1960    bl uuuu     u00u1 u    #d7 a10.1071/BI96915472DOI1 aLIGHTFOOT, R. J.  aFreezing ram spermatozoa by the pellet method. II. The effects of method of dilution, dilution rate, glycerol concentration, and duration of storage at 5oC prior to freezing on survival of spermatozoa.h[electronic resource]  c1960  aAbstract: Five factorial experiments were conducted to examine the relationship between the effects of diluent composition, method of dilution, dilution rate, glycerol con-centration, duration of storage at 5°C prior to freezing, and dilution rate at thawing on the survival of ram spermatozoa after freezing by the pellet method. The optimal method of dilution, glycerol concentration, and duration of storage were found to vary according to the composition of the diluent. With moderately hypertonic (6. = O· 7-0'9 degC) raffinose-yolk-citrate diluents, best post-thawing survival of spermatozoa was achieved by dilution with the glycerol-containing diluent at 30°C prior to cooling, with 3-4% glycerol in the diluted semen, and by a short (1 hr) period of storage at 5°C prior to pellet freezing.  aEmbryo transfer  aReproduction  aSheep  aSpermatozoa  aCongelamento  aEspermatozóide  aOvino  aReprodução  aSêmen  aSobrevivência  aDiluidor  aSemen diluents  aSemen preservation  aTecnologia do sêmen1 aSALAMON, S.  tAustralian Journal of Biological Sciencesgv. 22, n. 6, p. 547-560, 1960.