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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
01/08/1992 |
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Data da última atualização: |
05/07/2023 |
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Autoria: |
CHILLIARD, Y. |
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Título: |
Lipoprotein lipase of goat mammary tissue: comparison of extraction methods. |
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Ano de publicação: |
1979 |
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Fonte/Imprenta: |
Journal of Dairy Research, v. 46, p. 599-605, 1979. |
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DOI: |
10.1017/s0022029900020653 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Mammary lipoprotein lipase of lactating goats was extracted by 3 methods: homogenization of tissue acetone-ether powders; aqueous homogenization of crude tissue using an Ultra-Turrax apparatus; aqueous homogenization of crude tissue using a Sorvall Omni-mixer microhomogenizer. Although there were differences between absolute values obtained by the 3 methods, each type of homogenate had a lipolytic activity with lipoprotein lipase characteristics (i.e. more than 90% inhibition by serum omission or NaCl addition). Furthermore, the 3 methods were highly correlated and presented similar variations with the stage of lactation, in parallel with long-chain fatty acid secretion into milk. Repeatability of the measure of homogenate lipolytic activities was about 8%, whereas day-to-day repeatability of enzyme extraction and assay was about 20% for each method. |
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Thesagro: |
Caprino; Fisiologia; Reprodução. |
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Thesaurus Nal: |
Enzymology; Females; Goats; Lactating females; Mammary glands; Metabolism; Physiology; Reproduction. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 01594naa a2200265 a 4500
001 1520753
005 2023-07-05
008 1979 bl uuuu u00u1 u #d
024 7 $a10.1017/s0022029900020653$2DOI
100 1 $aCHILLIARD, Y.
245 $aLipoprotein lipase of goat mammary tissue$bcomparison of extraction methods.$h[electronic resource]
260 $c1979
520 $aAbstract: Mammary lipoprotein lipase of lactating goats was extracted by 3 methods: homogenization of tissue acetone-ether powders; aqueous homogenization of crude tissue using an Ultra-Turrax apparatus; aqueous homogenization of crude tissue using a Sorvall Omni-mixer microhomogenizer. Although there were differences between absolute values obtained by the 3 methods, each type of homogenate had a lipolytic activity with lipoprotein lipase characteristics (i.e. more than 90% inhibition by serum omission or NaCl addition). Furthermore, the 3 methods were highly correlated and presented similar variations with the stage of lactation, in parallel with long-chain fatty acid secretion into milk. Repeatability of the measure of homogenate lipolytic activities was about 8%, whereas day-to-day repeatability of enzyme extraction and assay was about 20% for each method.
650 $aEnzymology
650 $aFemales
650 $aGoats
650 $aLactating females
650 $aMammary glands
650 $aMetabolism
650 $aPhysiology
650 $aReproduction
650 $aCaprino
650 $aFisiologia
650 $aReprodução
773 $tJournal of Dairy Research$gv. 46, p. 599-605, 1979.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
