01594naa a2200265 a 450000100080000000500110000800800410001902400350006010000180009524501040011326000090021752008770022665000150110365000120111865000100113065000220114065000190116265000150118165000150119665000170121165000120122865000150124065000170125577300560127215207532023-07-05       1979    bl uuuu     u00u1 u    #d7 a10.1017/s00220299000206532DOI1 aCHILLIARD, Y.  aLipoprotein lipase of goat mammary tissuebcomparison of extraction methods.h[electronic resource]  c1979  aAbstract: Mammary lipoprotein lipase of lactating goats was extracted by 3 methods: homogenization of tissue acetone-ether powders; aqueous homogenization of crude tissue using an Ultra-Turrax apparatus; aqueous homogenization of crude tissue using a Sorvall Omni-mixer microhomogenizer. Although there were differences between absolute values obtained by the 3 methods, each type of homogenate had a lipolytic activity with lipoprotein lipase characteristics (i.e. more than 90% inhibition by serum omission or NaCl addition). Furthermore, the 3 methods were highly correlated and presented similar variations with the stage of lactation, in parallel with long-chain fatty acid secretion into milk. Repeatability of the measure of homogenate lipolytic activities was about 8%, whereas day-to-day repeatability of enzyme extraction and assay was about 20% for each method.  aEnzymology  aFemales  aGoats  aLactating females  aMammary glands  aMetabolism  aPhysiology  aReproduction  aCaprino  aFisiologia  aReprodução  tJournal of Dairy Researchgv. 46, p. 599-605, 1979.