| |
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Pecuária Sudeste. |
|
Data corrente: |
18/08/2005 |
|
Data da última atualização: |
08/05/2023 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
NOMURA, C. S.; SILVA, C. S.; NOGUEIRA, A. R. de A.; OLIVEIRA, P. V. |
|
Afiliação: |
CASSIANA S. NOMURA, Universidade de São Paulo; CINTIA S. SILVA, Universidade de São Paulo; ANA RITA DE ARAUJO NOGUEIRA, CPPSE; PEDRO V. OLIVEIRA, Universidade de São Paulo. |
|
Título: |
Bovine liver sample preparation and micro-homogeneity study for Cu and Zn determination by solid sampling electrothermal atomic absorption spectrometry. |
|
Ano de publicação: |
2005 |
|
Fonte/Imprenta: |
Spectrochimica Acta Part B, v. 60, p. 673-680, 2005. |
|
DOI: |
https://doi.org/10.1016/j.sab.2005.02.021 |
|
Idioma: |
Inglês |
|
Conteúdo: |
This work describes a systematic study for the bovine liver sample preparation for Cu and Zn determination by solid sampling electrothermal atomic absorption spectrometry. The main parameters investigated were sample drying, grinding process, particle size, sample size, microsample homogeneity, and their relationship with the precision and accuracy of the method. A bovine liver sample was prepared using different drying procedures: (1) freeze drying, and (2) drying in a household microwave oven followed by drying in a stove at 60 °C until constant mass. Ball and cryogenic mills were used for grinding. Less sensitive wavelengths for Cu (216.5 nm) and Zn (307.6 nm), and Zeeman-based three-field background correction for Cu were used to diminish the sensitivities. The pyrolysis and atomization temperatures adopted were 1000 °C and 2300 °C for Cu, and 700 °C and 1700 °C for Zn, respectively. For both elements, it was possible to calibrate the spectrometer with aqueous solutions. The use of 250 ?g of W + 200 ?g of Rh as permanent chemical modifier was imperative for Zn. Under these conditions, the characteristic mass and detection limit were 1.4 ng and 1.6 ng for Cu, and 2.8 ng and 1.3 ng for Zn, respectively. The results showed good agreement (95% confidence level) for homogeneity of the entire material (> 200 mg) when the sample was dried in microwave/stove and ground in a cryogenic mill. The microsample homogeneity study showed that Zn is more dependent on the sample pretreatment than Cu. The bovine liver sample prepared in microwave/stove and ground in a cryogenic mill presented results with the lowest relative standard deviation for Cu than Zn. Good accuracy and precision were observed for bovine liver masses higher than 40 ?g for Cu and 30 ?g for Zn. The concentrations of Cu and Zn in the prepared bovine liver sample were 223 mg kg? 1 and 128 mg kg? 1, respectively. The relative standard deviations were lower than 6% (n = 5). The accuracy of the entire procedure was checked with bovine liver from NIST (1577b) and determination of Cu and Zn using flame atomic absorption spectrometry after microwave-assisted sample digestion. MenosThis work describes a systematic study for the bovine liver sample preparation for Cu and Zn determination by solid sampling electrothermal atomic absorption spectrometry. The main parameters investigated were sample drying, grinding process, particle size, sample size, microsample homogeneity, and their relationship with the precision and accuracy of the method. A bovine liver sample was prepared using different drying procedures: (1) freeze drying, and (2) drying in a household microwave oven followed by drying in a stove at 60 °C until constant mass. Ball and cryogenic mills were used for grinding. Less sensitive wavelengths for Cu (216.5 nm) and Zn (307.6 nm), and Zeeman-based three-field background correction for Cu were used to diminish the sensitivities. The pyrolysis and atomization temperatures adopted were 1000 °C and 2300 °C for Cu, and 700 °C and 1700 °C for Zn, respectively. For both elements, it was possible to calibrate the spectrometer with aqueous solutions. The use of 250 ?g of W + 200 ?g of Rh as permanent chemical modifier was imperative for Zn. Under these conditions, the characteristic mass and detection limit were 1.4 ng and 1.6 ng for Cu, and 2.8 ng and 1.3 ng for Zn, respectively. The results showed good agreement (95% confidence level) for homogeneity of the entire material (> 200 mg) when the sample was dried in microwave/stove and ground in a cryogenic mill. The microsample homogeneity study showed that Zn is more dependent on the sample pretreatm... Mostrar Tudo |
|
Palavras-Chave: |
Bovine liver; Electrothermal atomic absorption spectrometry; Homogeneity; Sample preparation; Solid sampling. |
|
Categoria do assunto: |
W Química e Física |
|
Marc: |
LEADER 02967naa a2200229 a 4500
001 1047180
005 2023-05-08
008 2005 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.sab.2005.02.021$2DOI
100 1 $aNOMURA, C. S.
245 $aBovine liver sample preparation and micro-homogeneity study for Cu and Zn determination by solid sampling electrothermal atomic absorption spectrometry.$h[electronic resource]
260 $c2005
520 $aThis work describes a systematic study for the bovine liver sample preparation for Cu and Zn determination by solid sampling electrothermal atomic absorption spectrometry. The main parameters investigated were sample drying, grinding process, particle size, sample size, microsample homogeneity, and their relationship with the precision and accuracy of the method. A bovine liver sample was prepared using different drying procedures: (1) freeze drying, and (2) drying in a household microwave oven followed by drying in a stove at 60 °C until constant mass. Ball and cryogenic mills were used for grinding. Less sensitive wavelengths for Cu (216.5 nm) and Zn (307.6 nm), and Zeeman-based three-field background correction for Cu were used to diminish the sensitivities. The pyrolysis and atomization temperatures adopted were 1000 °C and 2300 °C for Cu, and 700 °C and 1700 °C for Zn, respectively. For both elements, it was possible to calibrate the spectrometer with aqueous solutions. The use of 250 ?g of W + 200 ?g of Rh as permanent chemical modifier was imperative for Zn. Under these conditions, the characteristic mass and detection limit were 1.4 ng and 1.6 ng for Cu, and 2.8 ng and 1.3 ng for Zn, respectively. The results showed good agreement (95% confidence level) for homogeneity of the entire material (> 200 mg) when the sample was dried in microwave/stove and ground in a cryogenic mill. The microsample homogeneity study showed that Zn is more dependent on the sample pretreatment than Cu. The bovine liver sample prepared in microwave/stove and ground in a cryogenic mill presented results with the lowest relative standard deviation for Cu than Zn. Good accuracy and precision were observed for bovine liver masses higher than 40 ?g for Cu and 30 ?g for Zn. The concentrations of Cu and Zn in the prepared bovine liver sample were 223 mg kg? 1 and 128 mg kg? 1, respectively. The relative standard deviations were lower than 6% (n = 5). The accuracy of the entire procedure was checked with bovine liver from NIST (1577b) and determination of Cu and Zn using flame atomic absorption spectrometry after microwave-assisted sample digestion.
653 $aBovine liver
653 $aElectrothermal atomic absorption spectrometry
653 $aHomogeneity
653 $aSample preparation
653 $aSolid sampling
700 1 $aSILVA, C. S.
700 1 $aNOGUEIRA, A. R. de A.
700 1 $aOLIVEIRA, P. V.
773 $tSpectrochimica Acta Part B$gv. 60, p. 673-680, 2005.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
Registro Completo
|
Biblioteca(s): |
Embrapa Amapá; Embrapa Tabuleiros Costeiros. |
|
Data corrente: |
30/01/2018 |
|
Data da última atualização: |
14/03/2018 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Circulação/Nível: |
Nacional - A |
|
Autoria: |
NEVES, M. S.; COUTO, M. V. S.; SOUSA, N. C.; SANTOS, R. F. B.; DIAS, H. M.; ABE, H. A.; DIAS, J. A. R.; CUNHA, F. S.; TAVARES-DIAS, M.; FUJIMOTO, R. Y. |
|
Afiliação: |
M. S. NEVES, UFPA; M. V. S. COUTO, UFPA; N. C. SOUSA, UFPA; R. F. B. SANTOS, UFPA; H. M. DIAS, UFPA; H. A. ABE, UFPA; J. A. R. DIAS, UFPA; F. S. CUNHA, Unifap; MARCOS TAVARES DIAS, CPAF-AP; RODRIGO YUDI FUJIMOTO, CPATC. |
|
Título: |
Resposta hematológica do cascudo ornamental amazônico Peckoltia oligospila ao estresse de transporte. |
|
Ano de publicação: |
2018 |
|
Fonte/Imprenta: |
Arquivo Brasileiro de Medicina Veterinária e Zootecnia, v. 70, n. 1, p. 13-19, 2018. |
|
Idioma: |
Português |
|
Conteúdo: |
O objetivo deste trabalho foi avaliar as respostas hematológicas do acari-bola Peckoltia oligospila submetido ao estresse de transporte. Variações nos parâmetros de sangue foram analisadas às zero, seis, 24, 48, 72 e 96 horas após o transporte. Respostas ao estresse foram observadas entre zero e seis horas do transporte, mas a maioria dos parâmetros retornou aos valores basais em 24 horas. O tempo de zero hora (momento imediato após transporte) foi o mais crítico, com valores elevados de glicemia, eritrócitos e eritroblastos. Respostas secundárias tardias foram observadas para a proteína plasmática total, o volume corpuscular médio (VCM) e a hemoglobina corpuscular média (HCM) em seis horas após o transporte dos peixes, retornando aos valores basais após esse período. O número de leucócitos não sofreu alterações após o transporte. O estresse de transporte não comprometeu a fisiologia de P. oligospila, o que indica que esse peixe é resistente ao estresse se comparado com outras espécies. Porém, recomenda-se que não se realize qualquer outro procedimento estressante durante pelo menos 24 horas da recuperação dos peixes após transporte, para garantir a saúde e a sobrevivência dos animais transportados. |
|
Palavras-Chave: |
Peixe endêmico. |
|
Thesagro: |
Biossegurança; Fisiologia animal; Sangue; Sanidade animal. |
|
Categoria do assunto: |
S Ciências Biológicas |
|
URL: |
https://www.alice.cnptia.embrapa.br/alice/bitstream/doc/1086654/1/CPAFAP2018Respostahematologicadocascudo.pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/171847/1/CPAF-AP-2018-Resposta-hematologica-do-cascudo.pdf
|
|
Marc: |
LEADER 02112naa a2200289 a 4500
001 2086654
005 2018-03-14
008 2018 bl uuuu u00u1 u #d
100 1 $aNEVES, M. S.
245 $aResposta hematológica do cascudo ornamental amazônico Peckoltia oligospila ao estresse de transporte.$h[electronic resource]
260 $c2018
520 $aO objetivo deste trabalho foi avaliar as respostas hematológicas do acari-bola Peckoltia oligospila submetido ao estresse de transporte. Variações nos parâmetros de sangue foram analisadas às zero, seis, 24, 48, 72 e 96 horas após o transporte. Respostas ao estresse foram observadas entre zero e seis horas do transporte, mas a maioria dos parâmetros retornou aos valores basais em 24 horas. O tempo de zero hora (momento imediato após transporte) foi o mais crítico, com valores elevados de glicemia, eritrócitos e eritroblastos. Respostas secundárias tardias foram observadas para a proteína plasmática total, o volume corpuscular médio (VCM) e a hemoglobina corpuscular média (HCM) em seis horas após o transporte dos peixes, retornando aos valores basais após esse período. O número de leucócitos não sofreu alterações após o transporte. O estresse de transporte não comprometeu a fisiologia de P. oligospila, o que indica que esse peixe é resistente ao estresse se comparado com outras espécies. Porém, recomenda-se que não se realize qualquer outro procedimento estressante durante pelo menos 24 horas da recuperação dos peixes após transporte, para garantir a saúde e a sobrevivência dos animais transportados.
650 $aBiossegurança
650 $aFisiologia animal
650 $aSangue
650 $aSanidade animal
653 $aPeixe endêmico
700 1 $aCOUTO, M. V. S.
700 1 $aSOUSA, N. C.
700 1 $aSANTOS, R. F. B.
700 1 $aDIAS, H. M.
700 1 $aABE, H. A.
700 1 $aDIAS, J. A. R.
700 1 $aCUNHA, F. S.
700 1 $aTAVARES-DIAS, M.
700 1 $aFUJIMOTO, R. Y.
773 $tArquivo Brasileiro de Medicina Veterinária e Zootecnia$gv. 70, n. 1, p. 13-19, 2018.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Amapá (CPAF-AP) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
