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Registro Completo |
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Biblioteca(s): |
Embrapa Instrumentação. |
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Data corrente: |
09/03/2005 |
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Data da última atualização: |
09/03/2005 |
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Autoria: |
OSIRO, D.; MUNIZ, J. R. C.; COLETA FILHO, H. D.; SOUSA, A. A. S.; MACHADO, M. A.; GARRATT, R. C.; COLNAGO, L. A. |
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Título: |
Fatty acid synthesis in Xylella fastidiosa: correlations between genome studies, 13C NMR data, and molecular models. |
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Ano de publicação: |
2004 |
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Fonte/Imprenta: |
Biochemical and Biophysical Research Communications, v. 323, p. 987-995, 2004. |
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Idioma: |
Inglês |
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Conteúdo: |
Xylella fastidiosa was the first plant pathogen to have its complete genome sequence elucidated. Routine database analyses suggested that two enzymes essential for fatty acid synthesis were missing, one of these is the holo-acyl-carrier-protein synthase. However, here we demonstrate, using 13C NMR spectroscopy, that X. fastidiosa is indeed able to synthesize fatty acids from acetate via an apparently conventional metabolic pathway. We further identify a gene product HetI, an alternative phosphopantetheinyl transferase, which we propose to fill the missing link. Homology modeling of HetI shows conservation of the Coenzyme A binding site suggesting it to be an active enzyme and reveals several interesting structural features when compared with the surfactin synthase activating enzyme, on which the model was built. These include a simplified topology due to N- and C-terminal deletions and lhe observation of a novel serine ladder. |
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Palavras-Chave: |
Modelagem molecular; NMR. |
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Thesagro: |
Síntese de Proteína; Xylella Fastidiosa. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01676naa a2200241 a 4500
001 1028851
005 2005-03-09
008 2004 bl --- 0-- u #d
100 1 $aOSIRO, D.
245 $aFatty acid synthesis in Xylella fastidiosa$bcorrelations between genome studies, 13C NMR data, and molecular models.
260 $c2004
520 $aXylella fastidiosa was the first plant pathogen to have its complete genome sequence elucidated. Routine database analyses suggested that two enzymes essential for fatty acid synthesis were missing, one of these is the holo-acyl-carrier-protein synthase. However, here we demonstrate, using 13C NMR spectroscopy, that X. fastidiosa is indeed able to synthesize fatty acids from acetate via an apparently conventional metabolic pathway. We further identify a gene product HetI, an alternative phosphopantetheinyl transferase, which we propose to fill the missing link. Homology modeling of HetI shows conservation of the Coenzyme A binding site suggesting it to be an active enzyme and reveals several interesting structural features when compared with the surfactin synthase activating enzyme, on which the model was built. These include a simplified topology due to N- and C-terminal deletions and lhe observation of a novel serine ladder.
650 $aSíntese de Proteína
650 $aXylella Fastidiosa
653 $aModelagem molecular
653 $aNMR
700 1 $aMUNIZ, J. R. C.
700 1 $aCOLETA FILHO, H. D.
700 1 $aSOUSA, A. A. S.
700 1 $aMACHADO, M. A.
700 1 $aGARRATT, R. C.
700 1 $aCOLNAGO, L. A.
773 $tBiochemical and Biophysical Research Communications$gv. 323, p. 987-995, 2004.
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Registro original: |
Embrapa Instrumentação (CNPDIA) |
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Biblioteca |
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Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
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URL |
Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Clima Temperado; Embrapa Hortaliças. |
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Data corrente: |
06/11/2019 |
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Data da última atualização: |
06/11/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
MARTINS, A. B. N.; CARVALHO, I. R.; COSTA, C. J.; MARQUES, F. S.; MARQUES, R. L. L.; XAVIER, F. Da M.; CAVALCANTE, J. A.; VERGARA, R. DE O.; TEIXEIRA, S. B.; BOHN, A.; TUNNES, L. V. M. DE; MACEDO, V. K.; REIS, B. B. DOS; SUNNE, A. DOS S.; MORAES, D. M. DE. |
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Afiliação: |
ANDRÉA BICCA NOGUEZ MARTINS, UFPEL; IVAN RICARDO CARVALHO, UFPEL; CAROLINE JACOME COSTA, CNPH; FERNANDA SEDREZ MARQUES, UFPEL; ROBSON LUIZ LEGÓRIO MARQUES, UFPEL; FERNANDA DA MOTTA XAVIER, UFPEL; JERFFESON ARAUJO CAVALCANTE; RAFAEL DE OLIVEIRA VERGARA, UFPEL; SHEILA BIGOLIN TEIXEIRA, UFPEL; ALBERTO BOHN, UFPEL; LILIAN VANUSSA MADRUGA DE TUNNES, UFPEL; VINICIUS KIESOW MACEDO, UFPEL; BRUNA BARRETO DOS REIS, UFPEL; ANNA DOS SANTOS SUNNE, UFPEL; DARIO MUNT DE MORAES, UFPEL. |
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Título: |
Storage of Amaranth Seeds: Reflex in Physiologic Potential. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Journal of Agricultural Science, v. 11, n. 12, 2019. |
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ISSN: |
1916-9752 |
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Idioma: |
Inglês |
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Conteúdo: |
The aimed to evaluate the effect of packages and different environment conditions in the maintenance of physiologic quality of Amaranthus cruentus seeds in the period of 300 days. The seeds used were from the cultivar BRS Alegria indicated for use in Brazilian winter. The experimental design completely randomized in factorial scheme (storage environment × package type × storage period) with four replicates. The amaranth seeds storage in plastic packages, in refrigerator and cold chamber, resulted in better germination maintenance ability. The conditioning in PET package, in natural and cold chamber environments promoted better results in relation to germination. The storage period did not present significant effect for the seeds stored in refrigerator and PET, although, for paper package there was reduction. The amaranth seeds, cv. BRS Alegria, can be stored in cold chamber for until 300 days, conditioned in PET, without reduction of its physiologic potential. |
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Thesagro: |
Amaranto de Grão; Pseudocereal; Semente. |
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Categoria do assunto: |
-- |
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URL: |
https://www.alice.cnptia.embrapa.br/alice/bitstream/doc/1113943/1/Storageofamaranthseedsreflexinphysiologicpotential.pdf
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Marc: |
LEADER 01899naa a2200337 a 4500
001 2113943
005 2019-11-06
008 2019 bl uuuu u00u1 u #d
022 $a1916-9752
100 1 $aMARTINS, A. B. N.
245 $aStorage of Amaranth Seeds$bReflex in Physiologic Potential.$h[electronic resource]
260 $c2019
520 $aThe aimed to evaluate the effect of packages and different environment conditions in the maintenance of physiologic quality of Amaranthus cruentus seeds in the period of 300 days. The seeds used were from the cultivar BRS Alegria indicated for use in Brazilian winter. The experimental design completely randomized in factorial scheme (storage environment × package type × storage period) with four replicates. The amaranth seeds storage in plastic packages, in refrigerator and cold chamber, resulted in better germination maintenance ability. The conditioning in PET package, in natural and cold chamber environments promoted better results in relation to germination. The storage period did not present significant effect for the seeds stored in refrigerator and PET, although, for paper package there was reduction. The amaranth seeds, cv. BRS Alegria, can be stored in cold chamber for until 300 days, conditioned in PET, without reduction of its physiologic potential.
650 $aAmaranto de Grão
650 $aPseudocereal
650 $aSemente
700 1 $aCARVALHO, I. R.
700 1 $aCOSTA, C. J.
700 1 $aMARQUES, F. S.
700 1 $aMARQUES, R. L. L.
700 1 $aXAVIER, F. Da M.
700 1 $aCAVALCANTE, J. A.
700 1 $aVERGARA, R. DE O.
700 1 $aTEIXEIRA, S. B.
700 1 $aBOHN, A.
700 1 $aTUNNES, L. V. M. DE
700 1 $aMACEDO, V. K.
700 1 $aREIS, B. B. DOS
700 1 $aSUNNE, A. DOS S.
700 1 $aMORAES, D. M. DE
773 $tJournal of Agricultural Science$gv. 11, n. 12, 2019.
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Registro original: |
Embrapa Clima Temperado (CPACT) |
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