01676naa a2200241 a 450000100080000000500110000800800410001910000140006024501210007426000090019552009450020465000260114965000230117565300240119865300080122270000200123070000240125070000200127470000190129470000190131370000190133277300830135110288512005-03-09       2004    bl ---       0--  u    #d1 aOSIRO, D.  aFatty acid synthesis in Xylella fastidiosabcorrelations between genome studies, 13C NMR data, and molecular models.  c2004  aXylella fastidiosa was the first plant pathogen to have its complete genome sequence elucidated. Routine database analyses suggested that two enzymes essential for fatty acid synthesis were missing, one of these is the holo-acyl-carrier-protein synthase. However, here we demonstrate, using 13C NMR spectroscopy, that X. fastidiosa is indeed able to synthesize fatty acids from acetate via an apparently conventional metabolic pathway. We further identify a gene product HetI, an alternative phosphopantetheinyl transferase, which we propose to fill the missing link. Homology modeling of HetI shows conservation of the Coenzyme A binding site suggesting it to be an active enzyme and reveals several interesting structural features when compared with the surfactin synthase activating enzyme, on which the model was built. These include a simplified topology due to N- and C-terminal deletions and lhe observation of a novel serine ladder.  aSíntese de Proteína  aXylella Fastidiosa  aModelagem molecular  aNMR1 aMUNIZ, J. R. C.1 aCOLETA FILHO, H. D.1 aSOUSA, A. A. S.1 aMACHADO, M. A.1 aGARRATT, R. C.1 aCOLNAGO, L. A.  tBiochemical and Biophysical Research Communicationsgv. 323, p. 987-995, 2004.