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Registro Completo |
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Biblioteca(s): |
Embrapa Roraima. |
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Data corrente: |
22/05/2023 |
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Data da última atualização: |
22/05/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
PANERO, F. dos P.; SMIDERLE, O. J.; PANERO, J. S.; FARIA, F. S. D. V; PANERO, P. dos S.; RODRIGUEZ, A. F. R. |
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Afiliação: |
FRANCISCO DOS SANTOS PANERO, UFRR; OSCAR JOSE SMIDERLE, CPAF-RR; JOÃO S. PANERO, IFRR; FERNANDO S. D. V. FARIA UNIVERSIDADE FEDERAL DO ACRE, UNIVERSIDADE FEDERAL DO ACRE; PEDRO DOS S. PANERO, IFRR; ANSELMO F. R. RODRIGUEZ, UNIVERSIDADE FEDERAL DO ACRE. |
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Título: |
Non-Destructive Genotyping of Cultivars and Strains of Sesame through NIR Spectroscopy and Chemometrics. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Biosensors, v. 12, n. 69, 2022. |
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Idioma: |
Inglês |
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Conteúdo: |
The differentiation of cultivars is carried out by means of morphological descriptors, in addition to molecular markers. In this work, near-infrared spectroscopy (NIR) and chemometric techniques were used to develop classification models for two different commercial sesame cultivars (Sesamum indicum) and 3 different strains. The diffuse reflectance spectra were recorded in the region of 700 to 2500 nm. Based on the application of chemometric techniques: principal component analysis?PCA, hierarchical cluster analysis?HCA, k-nearest neighbor?KNN and the flexible independent modeling of class analogy?SIMCA, from the infrared spectra in the near region, it was possible to perform the genotyping of two sesame cultivars (BRS Seda and BRS Anahí), and to classify these cultivars with 3 different sesame strains, obtaining 100% accurate results. Due to the good results obtained with the implemented models, the potential of the methods for a possible realization of forensic, fast and non-destructive authentication, in intact sesame seeds was evident. |
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Palavras-Chave: |
HCA; KNN; NIR; PCA; SIMCA. |
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Thesagro: |
Sesamum Indicum. |
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Categoria do assunto: |
-- |
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URL: |
https://www.alice.cnptia.embrapa.br/alice/bitstream/doc/1153868/1/artigoGergelimBIOSENSORS-1.pdf
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Marc: |
LEADER 01742naa a2200253 a 4500
001 2153868
005 2023-05-22
008 2022 bl uuuu u00u1 u #d
100 1 $aPANERO, F. dos P.
245 $aNon-Destructive Genotyping of Cultivars and Strains of Sesame through NIR Spectroscopy and Chemometrics.$h[electronic resource]
260 $c2022
520 $aThe differentiation of cultivars is carried out by means of morphological descriptors, in addition to molecular markers. In this work, near-infrared spectroscopy (NIR) and chemometric techniques were used to develop classification models for two different commercial sesame cultivars (Sesamum indicum) and 3 different strains. The diffuse reflectance spectra were recorded in the region of 700 to 2500 nm. Based on the application of chemometric techniques: principal component analysis?PCA, hierarchical cluster analysis?HCA, k-nearest neighbor?KNN and the flexible independent modeling of class analogy?SIMCA, from the infrared spectra in the near region, it was possible to perform the genotyping of two sesame cultivars (BRS Seda and BRS Anahí), and to classify these cultivars with 3 different sesame strains, obtaining 100% accurate results. Due to the good results obtained with the implemented models, the potential of the methods for a possible realization of forensic, fast and non-destructive authentication, in intact sesame seeds was evident.
650 $aSesamum Indicum
653 $aHCA
653 $aKNN
653 $aNIR
653 $aPCA
653 $aSIMCA
700 1 $aSMIDERLE, O. J.
700 1 $aPANERO, J. S.
700 1 $aFARIA, F. S. D. V
700 1 $aPANERO, P. dos S.
700 1 $aRODRIGUEZ, A. F. R.
773 $tBiosensors$gv. 12, n. 69, 2022.
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Registro original: |
Embrapa Roraima (CPAF-RR) |
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Registro Completo
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Biblioteca(s): |
Embrapa Pantanal. |
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Data corrente: |
06/12/2013 |
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Data da última atualização: |
15/04/2019 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
CAVALCANTE, R. V.; NOGUEIRA, M. F.; ARAÚJO Jr. J. P. |
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Afiliação: |
MARCIA FURLAN NOGUEIRA T DE LIMA, CPAP. |
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Título: |
Evaluation of nested and semi nested PCR in detection of equine infectious anemia virus infection in horses of Pantanal region, Brazil. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Virus Reviews & Research, v. 18, p. 219, 2013. Supplement 1. |
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Idioma: |
Inglês |
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Notas: |
Trabalho apresentado no XXIV Congresso Brasileiro de Virologia & VIII Reunião do Mercosul de Virologia, Porto Seguro, BA. |
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Conteúdo: |
Equine infectious anemia (EIA) is caused by a retrovirus and its primary mode of transmission comprises transfer of blood from infected animals to healthy ones. In Brazil, EIA notification is mandatory and its confirmation is based in serological tests followed by euthanasia of infected animals. AGID and ELISA are serological techniques recommended because have good accuracy and reliable results for detection of positive animals; however, some cases may display false results. Based on that, the aim of this study was to evaluate nested PCR technique in samples from Brazil. We tested five combinations of these primers, using 12 DNA samples extracted from positive horses from Pantanal (endemic area) in both serological techniques. |
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Palavras-Chave: |
Anemia Infecciosa Equina; EIA; EIAV (Equine infectious anemia virus); Retrovirus. |
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Thesagro: |
Eqüino; Virus. |
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Thesaurus NAL: |
Epidemiology; Equine infectious anemia; Horse diseases. |
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Categoria do assunto: |
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H Saúde e Patologia |
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URL: |
https://www.alice.cnptia.embrapa.br/alice/bitstream/doc/1076399/1/VV218.pdf
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Marc: |
LEADER 01639nam a2200253 a 4500
001 2076399
005 2019-04-15
008 2013 bl uuuu u00u1 u #d
100 1 $aCAVALCANTE, R. V.
245 $aEvaluation of nested and semi nested PCR in detection of equine infectious anemia virus infection in horses of Pantanal region, Brazil.
260 $aVirus Reviews & Research, v. 18, p. 219, 2013. Supplement 1.$c2013
500 $aTrabalho apresentado no XXIV Congresso Brasileiro de Virologia & VIII Reunião do Mercosul de Virologia, Porto Seguro, BA.
520 $aEquine infectious anemia (EIA) is caused by a retrovirus and its primary mode of transmission comprises transfer of blood from infected animals to healthy ones. In Brazil, EIA notification is mandatory and its confirmation is based in serological tests followed by euthanasia of infected animals. AGID and ELISA are serological techniques recommended because have good accuracy and reliable results for detection of positive animals; however, some cases may display false results. Based on that, the aim of this study was to evaluate nested PCR technique in samples from Brazil. We tested five combinations of these primers, using 12 DNA samples extracted from positive horses from Pantanal (endemic area) in both serological techniques.
650 $aEpidemiology
650 $aEquine infectious anemia
650 $aHorse diseases
650 $aEqüino
650 $aVirus
653 $aAnemia Infecciosa Equina
653 $aEIA
653 $aEIAV (Equine infectious anemia virus)
653 $aRetrovirus
700 1 $aNOGUEIRA, M. F.
700 1 $aARAÚJO Jr. J. P.
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Registro original: |
Embrapa Pantanal (CPAP) |
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