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 | Acesso ao texto completo restrito à biblioteca da Embrapa Arroz e Feijão. Para informações adicionais entre em contato com cnpaf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
13/12/2022 |
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Data da última atualização: |
13/12/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CÔRTES, M. V. de C. B.; BARRETO, M. S.; SILVA-LOBO, V. L. da; FREIRE, D. M. G. |
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Afiliação: |
MARCIO VINICIUS DE C BARROS CORTES, CNPAF; MAYSA SILVA BARRETO, UNIVERSIDADE FEDERAL DO RIO DE JANEIRO; VALACIA LEMES DA SILVA LOBO, CNPAF; DENISE MARIA GUIMARAES FREIRE, UNIVERSIDADE FEDERAL DO RIO DE JANEIRO. |
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Título: |
Sustainable production of biopesticides for common bean root rot control within the biorefinery approach: a Technology Readiness Level 3 experimental proof of concept. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Environmental Science and Pollution Research International, 2022. |
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ISSN: |
1614-7499 |
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DOI: |
https://doi.org/10.1007/s11356-022-24252-6 |
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Idioma: |
Inglês |
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Conteúdo: |
Biopesticides are recognized as an efficient alternative to synthetic pesticides for pest and disease crop management. However, their commercial production processes use grains, generating large amounts of organic waste, even when agriculture waste or byproducts are the feedstock of choice. Frequently, these organic wastes are rich in nutrients that, after adequate treatment, can be used as nitrogen and carbohydrate sources for secondary metabolite production produced by microorganisms during submerged fermentation. In this sense, this study aimed to prove the concept that biopesticides could be produced under a full biorefinery process, using the entire biomass of an underexplored agroindustrial waste-damaged bean-as the main feedstock. A combination of sequential processes, including solid state fermentation, hydrolysis, and submerged fermentation, were designed for the production of two biopesticides (conventional?fungal conidia and second-generation secondary metabolite-cerulenin) from a high potential biological control agent strain Sarocladium oryzae BRM 59907. The combined processes, using damaged common bean grain as the main feedstock, provided biopesticides and organic fertilizer production that successfully controlled common bean root rot disease. This work proved to be possible the biopesticide production using a full biorefinery concept, inside the same productive chain, contributing to a sustainable environment and economy, together with animal and human health safety. MenosBiopesticides are recognized as an efficient alternative to synthetic pesticides for pest and disease crop management. However, their commercial production processes use grains, generating large amounts of organic waste, even when agriculture waste or byproducts are the feedstock of choice. Frequently, these organic wastes are rich in nutrients that, after adequate treatment, can be used as nitrogen and carbohydrate sources for secondary metabolite production produced by microorganisms during submerged fermentation. In this sense, this study aimed to prove the concept that biopesticides could be produced under a full biorefinery process, using the entire biomass of an underexplored agroindustrial waste-damaged bean-as the main feedstock. A combination of sequential processes, including solid state fermentation, hydrolysis, and submerged fermentation, were designed for the production of two biopesticides (conventional?fungal conidia and second-generation secondary metabolite-cerulenin) from a high potential biological control agent strain Sarocladium oryzae BRM 59907. The combined processes, using damaged common bean grain as the main feedstock, provided biopesticides and organic fertilizer production that successfully controlled common bean root rot disease. This work proved to be possible the biopesticide production using a full biorefinery concept, inside the same productive chain, contributing to a sustainable environment and economy, together with animal and human health... Mostrar Tudo |
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Palavras-Chave: |
Biocontrol; Nutrient-rich waste hydrolysate. |
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Thesagro: |
Controle Biológico; Fermentação; Rhizoctonia Solani. |
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Thesaurus Nal: |
Biological control; Cerulenin; Fermentation; Sarocladium oryzae. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02488naa a2200289 a 4500
001 2149545
005 2022-12-13
008 2022 bl uuuu u00u1 u #d
022 $a1614-7499
024 7 $ahttps://doi.org/10.1007/s11356-022-24252-6$2DOI
100 1 $aCÔRTES, M. V. de C. B.
245 $aSustainable production of biopesticides for common bean root rot control within the biorefinery approach$ba Technology Readiness Level 3 experimental proof of concept.$h[electronic resource]
260 $c2022
520 $aBiopesticides are recognized as an efficient alternative to synthetic pesticides for pest and disease crop management. However, their commercial production processes use grains, generating large amounts of organic waste, even when agriculture waste or byproducts are the feedstock of choice. Frequently, these organic wastes are rich in nutrients that, after adequate treatment, can be used as nitrogen and carbohydrate sources for secondary metabolite production produced by microorganisms during submerged fermentation. In this sense, this study aimed to prove the concept that biopesticides could be produced under a full biorefinery process, using the entire biomass of an underexplored agroindustrial waste-damaged bean-as the main feedstock. A combination of sequential processes, including solid state fermentation, hydrolysis, and submerged fermentation, were designed for the production of two biopesticides (conventional?fungal conidia and second-generation secondary metabolite-cerulenin) from a high potential biological control agent strain Sarocladium oryzae BRM 59907. The combined processes, using damaged common bean grain as the main feedstock, provided biopesticides and organic fertilizer production that successfully controlled common bean root rot disease. This work proved to be possible the biopesticide production using a full biorefinery concept, inside the same productive chain, contributing to a sustainable environment and economy, together with animal and human health safety.
650 $aBiological control
650 $aCerulenin
650 $aFermentation
650 $aSarocladium oryzae
650 $aControle Biológico
650 $aFermentação
650 $aRhizoctonia Solani
653 $aBiocontrol
653 $aNutrient-rich waste hydrolysate
700 1 $aBARRETO, M. S.
700 1 $aSILVA-LOBO, V. L. da
700 1 $aFREIRE, D. M. G.
773 $tEnvironmental Science and Pollution Research International, 2022.
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Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
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Registro Completo
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
02/02/2023 |
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Data da última atualização: |
24/03/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
VANEGAS K. G.; RENDSVIG, J. K. H.; JARCZYSKA, Z. D.; CÔRTES, M. V. de C. B.; VAN ESCH, A. P.; MORERA-GÓMEZ, M.; CONTESINI, F. J.; MORTENSEN, U. H. |
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Afiliação: |
KATHERINA GARCIA VANEGAS, UNIVERSITY OF DENAMARK; JAKOB KRÆMMER HAAR RENDSVIG, UNIVERSITY OF DENMARK; ZOFIA DOROTA JARCZYNSKA, UNIVERSITY OF DENMARK; MARCIO VINICIUS DE C BARROS CORTES, CNPAF; ABEL PETER VAN ESCH, UNIVERSITY OF DENAMARK; MARTÍ MORERA-GÓMEZ, UNIVERSITY OF DENAMARK; FABIANO JARES CONTESINI, UNIVERSITY OF DENAMARK; UFFE HASBRO MORTENSEN, UNIVERSITY OF DENAMARK. |
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Título: |
A Mad7 system for genetic engineering of filamentous fungi. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Journal of Fungi, v. 9, n. 1, 16, 2023. |
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ISSN: |
2309-608X |
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DOI: |
https://doi.org/10.3390/jof9010016 |
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Idioma: |
Inglês |
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Conteúdo: |
The introduction of CRISPR technologies has revolutionized strain engineering in filamentous fungi. However, its use in commercial applications has been hampered by concerns over intellectual property (IP) ownership, and there is a need for implementing Cas nucleases that are not limited by complex IP constraints. One promising candidate in this context is the Mad7 enzyme, and we here present a versatile Mad7-CRISPR vector-set that can be efficiently used for the genetic engineering of four different Aspergillus species: Aspergillus nidulans, A. niger, A. oryzae and A. campestris, the latter being a species that has never previously been genetically engineered. We successfully used Mad7 to introduce unspecific as well as specific template-directed mutations including gene disruptions, gene insertions and gene deletions. Moreover, we demonstrate that both single-stranded oligonucleotides and PCR fragments equipped with short and long targeting sequences can be used for efficient marker-free gene editing. Importantly, our CRISPR/Mad7 system was functional in both non-homologous end-joining (NHEJ) proficient and deficient strains. Therefore, the newly implemented CRISPR/Mad7 was efficient to promote gene deletions and integrations using different types of DNA repair in four different Aspergillus species, resulting in the expansion of CRISPR toolboxes in fungal cell factories. |
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Palavras-Chave: |
CRISPR; Filamentous fungi; Fungal strain engineering; Mad7. |
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Thesagro: |
Fungo. |
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Thesaurus NAL: |
Aspergillus; Fungi; Genetic engineering. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1151460/1/jof-2023.pdf
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Marc: |
LEADER 02290naa a2200325 a 4500
001 2151460
005 2023-03-24
008 2023 bl uuuu u00u1 u #d
022 $a2309-608X
024 7 $ahttps://doi.org/10.3390/jof9010016$2DOI
100 1 $aVANEGAS K. G.
245 $aA Mad7 system for genetic engineering of filamentous fungi.$h[electronic resource]
260 $c2023
520 $aThe introduction of CRISPR technologies has revolutionized strain engineering in filamentous fungi. However, its use in commercial applications has been hampered by concerns over intellectual property (IP) ownership, and there is a need for implementing Cas nucleases that are not limited by complex IP constraints. One promising candidate in this context is the Mad7 enzyme, and we here present a versatile Mad7-CRISPR vector-set that can be efficiently used for the genetic engineering of four different Aspergillus species: Aspergillus nidulans, A. niger, A. oryzae and A. campestris, the latter being a species that has never previously been genetically engineered. We successfully used Mad7 to introduce unspecific as well as specific template-directed mutations including gene disruptions, gene insertions and gene deletions. Moreover, we demonstrate that both single-stranded oligonucleotides and PCR fragments equipped with short and long targeting sequences can be used for efficient marker-free gene editing. Importantly, our CRISPR/Mad7 system was functional in both non-homologous end-joining (NHEJ) proficient and deficient strains. Therefore, the newly implemented CRISPR/Mad7 was efficient to promote gene deletions and integrations using different types of DNA repair in four different Aspergillus species, resulting in the expansion of CRISPR toolboxes in fungal cell factories.
650 $aAspergillus
650 $aFungi
650 $aGenetic engineering
650 $aFungo
653 $aCRISPR
653 $aFilamentous fungi
653 $aFungal strain engineering
653 $aMad7
700 1 $aRENDSVIG, J. K. H.
700 1 $aJARCZYSKA, Z. D.
700 1 $aCÔRTES, M. V. de C. B.
700 1 $aVAN ESCH, A. P.
700 1 $aMORERA-GÓMEZ, M.
700 1 $aCONTESINI, F. J.
700 1 $aMORTENSEN, U. H.
773 $tJournal of Fungi$gv. 9, n. 1, 16, 2023.
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