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 | Acesso ao texto completo restrito à biblioteca da Embrapa Trigo. Para informações adicionais entre em contato com cnpt.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia; Embrapa Trigo. |
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Data corrente: |
21/02/2022 |
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Data da última atualização: |
05/09/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ROESLER, E. A.; BALBINOTT, N.; SCHROEDER, R. G.; LAU, E. Y.; LIMA, M. I. P. M.; BRAMMER, S. P.; ARAGÃO, F. J. L.; FERNANDES, J. M. C.; DEUNER, C. C. |
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Afiliação: |
EDUARDO ANDRÉ ROESLER, UPF; NATALIA BALBINOTT, UFRGS; RENATA GABRIELA SCHROEDER, UPF; ELENE YAMAZAKI LAU, CNPT; MARIA IMACULADA PONTES M LIMA, CNPT; SANDRA PATUSSI BRAMMER, CNPT; FRANCISCO JOSE LIMA ARAGAO, Cenargen; JOSE MAURICIO CUNHA FERNANDES, CNPT; CAROLINA CARDOSO DEUNER, UPF. |
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Título: |
An efficient protocol for inoculation of Fusarium graminearum in detached leaves of Arabidopsis. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Tropical Plant Pathology, 16 Feb. 2022 |
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DOI: |
https://doi.org/10.1007/s40858-022-00497-x |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Fusarium graminearum is the main cause of Fusarium head blight, a fungal disease that reduces yield and afects the quality of wheat and other small grains. Genetic resistance is the ideal method for disease control; however, there are not yet cultivars with sufcient resistance levels to withstand an epidemic. Genetic engineering strategies such as gene silencing, host-induced gene silencing (HIGS), overexpression, and genome editing are promising, but many target genes need to be analyzed to fnd a suitable one. Arabidopsis thaliana is a model plant that is also susceptible to F. graminearum. Although this interaction was reported nearly two decades ago, consistent infection and symptoms are not always obtained. The availability of an efcient inoculation method is essential for studying plant-pathogen interaction. This work aimed at testing protocols for inoculating F. graminearum in detached leaves of A. thaliana which varied in inoculation site (leaf sides), wound (abrasion or hole injury), and inoculum type (mycelium agar disk or spore). We found that a mycelium agar disk placed on an abrasion injury at the adaxial leaf side was the most efcient (highest lesion size, incidence, and easiness to visualize the symptoms caused by the pathogen) method to inoculate F. graminearum in detached leaves of Arabidopsis. |
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Palavras-Chave: |
Inoculation assay; Protocol. |
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Thesagro: |
Doença Fúngica; Ferrugem; Fusarium Graminearum; Trigo. |
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Thesaurus Nal: |
Arabidopsis thaliana; Fusarium head blight. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02292naa a2200325 a 4500
001 2140281
005 2022-09-05
008 2022 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s40858-022-00497-x$2DOI
100 1 $aROESLER, E. A.
245 $aAn efficient protocol for inoculation of Fusarium graminearum in detached leaves of Arabidopsis.$h[electronic resource]
260 $c2022
520 $aAbstract: Fusarium graminearum is the main cause of Fusarium head blight, a fungal disease that reduces yield and afects the quality of wheat and other small grains. Genetic resistance is the ideal method for disease control; however, there are not yet cultivars with sufcient resistance levels to withstand an epidemic. Genetic engineering strategies such as gene silencing, host-induced gene silencing (HIGS), overexpression, and genome editing are promising, but many target genes need to be analyzed to fnd a suitable one. Arabidopsis thaliana is a model plant that is also susceptible to F. graminearum. Although this interaction was reported nearly two decades ago, consistent infection and symptoms are not always obtained. The availability of an efcient inoculation method is essential for studying plant-pathogen interaction. This work aimed at testing protocols for inoculating F. graminearum in detached leaves of A. thaliana which varied in inoculation site (leaf sides), wound (abrasion or hole injury), and inoculum type (mycelium agar disk or spore). We found that a mycelium agar disk placed on an abrasion injury at the adaxial leaf side was the most efcient (highest lesion size, incidence, and easiness to visualize the symptoms caused by the pathogen) method to inoculate F. graminearum in detached leaves of Arabidopsis.
650 $aArabidopsis thaliana
650 $aFusarium head blight
650 $aDoença Fúngica
650 $aFerrugem
650 $aFusarium Graminearum
650 $aTrigo
653 $aInoculation assay
653 $aProtocol
700 1 $aBALBINOTT, N.
700 1 $aSCHROEDER, R. G.
700 1 $aLAU, E. Y.
700 1 $aLIMA, M. I. P. M.
700 1 $aBRAMMER, S. P.
700 1 $aARAGÃO, F. J. L.
700 1 $aFERNANDES, J. M. C.
700 1 $aDEUNER, C. C.
773 $tTropical Plant Pathology, 16 Feb. 2022
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Registro original: |
Embrapa Trigo (CNPT) |
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| Registros recuperados : 5 | |
| 1. |  | ROESLER, E. A.; BALBINOTT, N.; SCHROEDER, R. G.; LAU, E. Y.; LIMA, M. I. P. M.; BRAMMER, S. P.; ARAGÃO, F. J. L.; FERNANDES, J. M. C.; DEUNER, C. C. An efficient protocol for inoculation of Fusarium graminearum in detached leaves of Arabidopsis. Tropical Plant Pathology, 16 Feb. 2022| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia; Embrapa Trigo. |
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| 2. | | ROESLER, E. A.; BALBINOTT, N.; YAMAZAKI-LAU, E.; LIMA, M. I. P. M.; DEUNER, C. C.; TIBOLA, C. S.; FERNANDES, J. M. C. Avaliação de Arabidopsis thaliana contendo a construção HIGS-CYP51-TRI mediante inoculação com Fusarium graminearum. In: MOSTRA DE INICIAÇÃO CIENTÍFICA, 13.; MOSTRA DE PÓS-GRADUAÇÃO DA EMBRAPA TRIGO, 10., 2018, Passo Fundo. Resumos... Brasília, DF: Embrapa, 2018. p. 45. Pós-graduação.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Trigo. |
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| 3. | | BALBINOTT, N.; ROESLER, E. A.; YAMAZAKI-LAU, E.; LIMA, M. I. P. M.; BONATO, A. L. V.; TIBOLA, C. S.; FERNANDES, J. M. C. Construção HIGS RNAI-CHS3B em Arabidopsis thaliana visando resistência a Fusarium graminearum. In: MOSTRA DE INICIAÇÃO CIENTÍFICA, 13.; MOSTRA DE PÓS-GRADUAÇÃO DA EMBRAPA TRIGO, 10., 2018, Passo Fundo. Resumos... Brasília, DF: Embrapa, 2018. p. 23. Resumos Graduação Pibic/CNPq.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Trigo. |
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| 4. | | ESPÍNDOLA, J. P.; BALBINOTT, N.; GRESSLER, L. T.; MACHADO, G.; KLEIN, C. S.; REBELATTO, R.; MARTÍN, C. B. G.; KREUTZ, L. C.; SCHRYVERS, A. B.; FRANDOLOSO, R. Molecular serotyping of clinical strains of Haemophilus (Glaesserella) parasuis brings new insights regarding Glassers disease outbreaks in Brazil. PeerJ, v. 7, n. e6817, 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 5. | | BALBINOTT, N.; ROESLER, E. A.; YAMAZAKI-LAU, E.; LIMA, M. I. P. M.; BONATO, A. L. V.; FERNANDES, J. M. C. Resistência a Fusarium graminearum via silenciamento gênico induzido pelo hospedeiro de Chs3b em Arabidopsis thaliana. In: MOSTRA DE INICIAÇÃO CIENTÍFICA, 12.; MOSTRA DE PÓS-GRADUAÇÃO DA EMBRAPA TRIGO, 9., 2017, Passo Fundo. Resumos... Passo Fundo: Embrapa Trigo, 2017. p. 27.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Trigo. |
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| Registros recuperados : 5 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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