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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
17/11/2020 |
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Data da última atualização: |
17/11/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MOURA, A. G. L.; RABELO, C. A. B. S.; OKINO, C. H.; MAINTINGUER, S. I.; SILVA, E. L.; VARESCHE, M. B. A. |
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Afiliação: |
A. G .L. MOURA, USP; C. A. B. S. Rabelo, USP; CINTIA HIROMI OKINO, CPPSE; S. I. MAINTINGUER, UNESP; E. L. SILVA, UFSCAR; M. B. A. VARESCHE, USP. |
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Título: |
Enhancement of Clostridium butyricum hydrogen production by iron and nickel nanoparticles: effects on hydA expression. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
International Journal of Hydrogen Energy, v. 45, n. 53, p. 28447-28431, oct. 2020. |
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DOI: |
https://doi.org/10.1016/j.ijhydene.2020.07.161 |
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Idioma: |
Inglês |
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Conteúdo: |
In this study, an optimized medium for Clostridium butyricum (CCT7470) was obtained from a Plackett-Burman assay. It was evidenced that glucose (5 g/L), starch (1 g/L) and three nitrogen and vitamin sources (5 g meat extract/L; 5 g peptone/L and 1.5 g yeast extract/L) improved hydrogen (H2) production. The addition of nanoparticles (NPs) improved the fermentation process, and maximum H2 production (434 ± 13 mL) was achieved with 30 mg Fe0/L and 20 mg Ni0/L. The metabolic pathway shifted from acetic and butyric to valeric acid production with the addition of NPs. In reactors with NPs, a longer permanence of hydrogenase was observed with hydA quantification by real-time polymerase chain reaction (qPCR). However, the expression of new enzymes, assessed by reverse transcription followed by qPCR (RT-qPCR), was higher in control assay than that in the NPs one. |
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Palavras-Chave: |
Dark fermentation; Iron nanoparticles; Nickel nanoparticles. |
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Thesaurus Nal: |
Clostridium butyricum; Response surface methodology. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 01737naa a2200253 a 4500
001 2126683
005 2020-11-17
008 2020 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.ijhydene.2020.07.161$2DOI
100 1 $aMOURA, A. G. L.
245 $aEnhancement of Clostridium butyricum hydrogen production by iron and nickel nanoparticles$beffects on hydA expression.$h[electronic resource]
260 $c2020
520 $aIn this study, an optimized medium for Clostridium butyricum (CCT7470) was obtained from a Plackett-Burman assay. It was evidenced that glucose (5 g/L), starch (1 g/L) and three nitrogen and vitamin sources (5 g meat extract/L; 5 g peptone/L and 1.5 g yeast extract/L) improved hydrogen (H2) production. The addition of nanoparticles (NPs) improved the fermentation process, and maximum H2 production (434 ± 13 mL) was achieved with 30 mg Fe0/L and 20 mg Ni0/L. The metabolic pathway shifted from acetic and butyric to valeric acid production with the addition of NPs. In reactors with NPs, a longer permanence of hydrogenase was observed with hydA quantification by real-time polymerase chain reaction (qPCR). However, the expression of new enzymes, assessed by reverse transcription followed by qPCR (RT-qPCR), was higher in control assay than that in the NPs one.
650 $aClostridium butyricum
650 $aResponse surface methodology
653 $aDark fermentation
653 $aIron nanoparticles
653 $aNickel nanoparticles
700 1 $aRABELO, C. A. B. S.
700 1 $aOKINO, C. H.
700 1 $aMAINTINGUER, S. I.
700 1 $aSILVA, E. L.
700 1 $aVARESCHE, M. B. A.
773 $tInternational Journal of Hydrogen Energy$gv. 45, n. 53, p. 28447-28431, oct. 2020.
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| Registros recuperados : 7 | |
| 1. |  | ANDRIOLI, A.; SIMPLICIO, A. A.; VISINTIN, J. A.; SOARES, A. T. Resposta superovulatória em caprinos após repetidos tratamentos com gonadotrofina hipofisária suína. ARS Veterinaria, Jaboticabal, v. 11, n. 2, p. 116-117, 1995. Anais da 10 Reunião anual da Sociedade Brasileira de Transferência de Embriões, 1995.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 4. | | SIMÕES, R; NICACIO, A. C.; BINELLI, M.; LOPES, F. F. de P.; MILAZZOTTO, M. P.; VISINTIN, J. A.; D'ÁVILA ASSUMPÇÃO, M. E. O. Sperm-mediated gene transfer: effect on bovine in vitro embryo production. Zygote, october, p. 1-5, 2012. doi:10.1017/S0967199412000147| Biblioteca(s): Embrapa Gado de Corte. |
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| 7. | | MIGLINO, M. A.; PEREIRA, F. T. V.; VISINTIN, J. A.; GARCIA, J. M.; MEIRELLES, F. V.; RUMPF, R.; AMBRÓSIO, C. E.; PAPA, P. C.; SANTOS, T. C.; CARVALHO, A. F.; LEISER, R.; CARTER, A. M. Placentation in cloned cattle: structure and microvascular architecture. Theriogenology, v. 68, p. 604-617, 2007.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| Registros recuperados : 7 | |
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