01737naa a2200253 a 450000100080000000500110000800800410001902400560006010000200011624501460013626000090028252008720029165000260116365000330118965300220122265300230124465300250126770000240129270000170131670000230133370000170135670000230137377300870139621266832020-11-17       2020    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.1016/j.ijhydene.2020.07.1612DOI1 aMOURA, A. G. L.  aEnhancement of Clostridium butyricum hydrogen production by iron and nickel nanoparticlesbeffects on hydA expression.h[electronic resource]  c2020  aIn this study, an optimized medium for Clostridium butyricum (CCT7470) was obtained from a Plackett-Burman assay. It was evidenced that glucose (5 g/L), starch (1 g/L) and three nitrogen and vitamin sources (5 g meat extract/L; 5 g peptone/L and 1.5 g yeast extract/L) improved hydrogen (H2) production. The addition of nanoparticles (NPs) improved the fermentation process, and maximum H2 production (434 ± 13 mL) was achieved with 30 mg Fe0/L and 20 mg Ni0/L. The metabolic pathway shifted from acetic and butyric to valeric acid production with the addition of NPs. In reactors with NPs, a longer permanence of hydrogenase was observed with hydA quantification by real-time polymerase chain reaction (qPCR). However, the expression of new enzymes, assessed by reverse transcription followed by qPCR (RT-qPCR), was higher in control assay than that in the NPs one.  aClostridium butyricum  aResponse surface methodology  aDark fermentation  aIron nanoparticles  aNickel nanoparticles1 aRABELO, C. A. B. S.1 aOKINO, C. H.1 aMAINTINGUER, S. I.1 aSILVA, E. L.1 aVARESCHE, M. B. A.  tInternational Journal of Hydrogen Energygv. 45, n. 53, p. 28447-28431, oct. 2020.