| |
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Semiárido. Para informações adicionais entre em contato com cpatsa.biblioteca@embrapa.br. |
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Semiárido. |
|
Data corrente: |
19/02/2019 |
|
Data da última atualização: |
21/01/2020 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
SILVA, V. B. da; SILVA, A. F. da; SILVA, T. R. da; SANTOS, J. W. M. dos; SILVA, J. F. da; SOUZA, A. P. de; FREITAS, A. D. S. de; FERNANDES JUNIOR, P. I. |
|
Afiliação: |
VALÉRIA BORGES DA SILVA; ALEKSANDRO FERREIRA DA SILVA; THAISE ROSA DA SILVA; JONNATHAN WHINY MORAES DOS SANTOS; JÉSSICA FERNANDA DA SILVA; ADAILSON PEREIRA DE SOUZA; ANA DOLORES SANTIAGO DE FREITAS; PAULO IVAN FERNANDES JUNIOR, CPATSA. |
|
Título: |
Fast and efficient symbiotic gene-based duplex PCR approach for the preliminary selection of legume root nodule bacteria. |
|
Ano de publicação: |
2019 |
|
Fonte/Imprenta: |
Rhizosphere, v. 10, 2019. |
|
ISSN: |
2452-2198 |
|
DOI: |
10.1016/j.rhisph.2019.100144 |
|
Idioma: |
Inglês |
|
Conteúdo: |
The isolation of root nodule bacteria (RNB) usually lead to obtainment of several non-rhizobia, mainly those fastgrowing bacteria. The plant-authentication experiments to select the nodulating bacteria are time consuming and can be ruined in case of nodulation of negative controls. To speed up this step in rhizobiology research a fast, simple and efficient duplex PCR assay for the amplification of nodC and nifH genes in was developed aiming to separate rhizobia and non-rhizobia in RNB culture collections. The method was optimized with 43 reference strains and was applied to a new culture collection for validation. Considering all bacteria, 106 out of 109 nodulating rhizobia were positive for both genes. Among non-nodulating, 48 out of 48 were nodC-negative. The results demonstrated the efficiency of this new method that can be completed in a day, to save time and money in RNB isolation projects, fulfilling Koch's postulate, and separating rhizobia from non-rhizobial bacteria. |
|
Palavras-Chave: |
Koch's postulates; Nódulo da raiz. |
|
Thesagro: |
Bactéria; Leguminosa. |
|
Thesaurus Nal: |
Legumes. |
|
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
|
Marc: |
LEADER 01846naa a2200289 a 4500
001 2106246
005 2020-01-21
008 2019 bl uuuu u00u1 u #d
022 $a2452-2198
024 7 $a10.1016/j.rhisph.2019.100144$2DOI
100 1 $aSILVA, V. B. da
245 $aFast and efficient symbiotic gene-based duplex PCR approach for the preliminary selection of legume root nodule bacteria.$h[electronic resource]
260 $c2019
520 $aThe isolation of root nodule bacteria (RNB) usually lead to obtainment of several non-rhizobia, mainly those fastgrowing bacteria. The plant-authentication experiments to select the nodulating bacteria are time consuming and can be ruined in case of nodulation of negative controls. To speed up this step in rhizobiology research a fast, simple and efficient duplex PCR assay for the amplification of nodC and nifH genes in was developed aiming to separate rhizobia and non-rhizobia in RNB culture collections. The method was optimized with 43 reference strains and was applied to a new culture collection for validation. Considering all bacteria, 106 out of 109 nodulating rhizobia were positive for both genes. Among non-nodulating, 48 out of 48 were nodC-negative. The results demonstrated the efficiency of this new method that can be completed in a day, to save time and money in RNB isolation projects, fulfilling Koch's postulate, and separating rhizobia from non-rhizobial bacteria.
650 $aLegumes
650 $aBactéria
650 $aLeguminosa
653 $aKoch's postulates
653 $aNódulo da raiz
700 1 $aSILVA, A. F. da
700 1 $aSILVA, T. R. da
700 1 $aSANTOS, J. W. M. dos
700 1 $aSILVA, J. F. da
700 1 $aSOUZA, A. P. de
700 1 $aFREITAS, A. D. S. de
700 1 $aFERNANDES JUNIOR, P. I.
773 $tRhizosphere$gv. 10, 2019.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Semiárido (CPATSA) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
| Registros recuperados : 25 | |
| 10. |  | AVIDOS, M. F. D. (Org.). Cenargenda online. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2007. 1 CD-ROM. (Embrapa Recursos Genéticos e Biotecnologia. Documentos, 247).| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
|   |
| 20. | | FOLLE, S. M.; FERREIRA, M. A.; FORTES, L. S.; AVIDOS, M. F. D.; CAJUEIRO, E. V. de M.; CONTE, A. C.; TIGANO, M. S.; MACHADO, M. I. P. Atividades do Núcleo de Gestão da Informação da Embrapa Recursos Genéticos e Biotecnologia. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2008. 25 p. (Embrapa Recursos Genéticos e Biotecnologia. Documentos, 267).| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
|  |
| Registros recuperados : 25 | |
|
| Expressão de busca inválida. Verifique!!! |
|
|
