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Registro Completo |
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Biblioteca(s): |
Embrapa Pesca e Aquicultura. |
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Data corrente: |
17/07/2017 |
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Data da última atualização: |
17/07/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
LUIZ, D. de B.; SILVA, C. D. F. e; CAMPELO, S. R.; SANTOS, V. R. V. dos; LIMA, L. K. F. de; CHICRALA, P. C. M. S.; IWASHITA, M. K. P. |
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Afiliação: |
DANIELLE DE BEM LUIZ, CNPASA; CARLOS DANGER FERREIRA E SILVA; SIMONE RODRIGUES CAMPELO, CNPASA; VIVIANE RODRIGUES V DOS SANTOS, CNPASA; LEANDRO KANAMARU FRANCO DE LIMA, CNPASA; PATRICIA COSTA M SOARES CHICRALA, CNPASA; MARINA KEIKO PIERONI IWASHITA, SNE. |
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Título: |
Evaluation of the effectiveness of ozone as a sanitizer for fish experimentally contaminated with Salmonella sp. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Brazilian Journal of Food Technology, Campinas, v. 20, e2016150, 2017. |
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ISSN: |
1981-6723 |
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DOI: |
10.1590/1981-6723.15016 |
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Idioma: |
Inglês |
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Conteúdo: |
Salmonellosis is a major public health problem related to food contamination and ensuing food poisoning. Brazilian resolution RDC nº 12/2001 of the Brazilian National Health Surveillance Agency (ANVISA) established the absence of Salmonella in 25 g of fish for consumption. However, the significant increase in the occurrence of fish contamination by Salmonella and other pathogenic bacteria shows that the currently applied strategies are not sufficient and that, in addition to the implementation of good health practices, the application of new sanitizer technologies in the fish industry is also necessary. In this context, the present study evaluated the effectiveness of ozone in an aqueous medium as a sanitizer for Salmonella contaminated fish. The experiment was carried out using a completely randomized design with eight treatments and five replicates, giving a total of 40 experimental units. Each sample consisted of three fishes, totalizing 120 fishes. The treatments consisted of different combinations of temperature and water-dissolved ozone (O3) concentrations (21 °C × 0.35 ppm; 20 °C × 0.45 ppm; 21 °C × 0.60 ppm; 20 °C × 0.80 ppm; 19 °C × 1.7 ppm; 6 × 5.1 ppm; 4 °C × 7.2 ppm; and 2 °C × 9.1 ppm). Colossoma macropomum (Tambaqui) samples were experimentally infected with Salmonella typhymurium (ATCC 14028) and immersed in water with the different treatments. After three minutes, the fish samples were collected and subjected to qualitative Salmonella analyses. The ozone tests were not efficient in eradicating Salmonella under the experimental conditions presented here, indicating the need for the identification of effective sanitizers in order to meet the determinations of Brazilian law. MenosSalmonellosis is a major public health problem related to food contamination and ensuing food poisoning. Brazilian resolution RDC nº 12/2001 of the Brazilian National Health Surveillance Agency (ANVISA) established the absence of Salmonella in 25 g of fish for consumption. However, the significant increase in the occurrence of fish contamination by Salmonella and other pathogenic bacteria shows that the currently applied strategies are not sufficient and that, in addition to the implementation of good health practices, the application of new sanitizer technologies in the fish industry is also necessary. In this context, the present study evaluated the effectiveness of ozone in an aqueous medium as a sanitizer for Salmonella contaminated fish. The experiment was carried out using a completely randomized design with eight treatments and five replicates, giving a total of 40 experimental units. Each sample consisted of three fishes, totalizing 120 fishes. The treatments consisted of different combinations of temperature and water-dissolved ozone (O3) concentrations (21 °C × 0.35 ppm; 20 °C × 0.45 ppm; 21 °C × 0.60 ppm; 20 °C × 0.80 ppm; 19 °C × 1.7 ppm; 6 × 5.1 ppm; 4 °C × 7.2 ppm; and 2 °C × 9.1 ppm). Colossoma macropomum (Tambaqui) samples were experimentally infected with Salmonella typhymurium (ATCC 14028) and immersed in water with the different treatments. After three minutes, the fish samples were collected and subjected to qualitative Salmonella analyses. The ozone test... Mostrar Tudo |
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Thesagro: |
Colossoma macropomum; Microbiologia; Ozônio; Peixe de água doce; Salmonella; Tambaqui. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://www.alice.cnptia.embrapa.br/alice/bitstream/doc/1072729/1/CNPASA2017bjft.pdf
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Marc: |
LEADER 02621naa a2200289 a 4500
001 2072729
005 2017-07-17
008 2017 bl uuuu u00u1 u #d
022 $a1981-6723
024 7 $a10.1590/1981-6723.15016$2DOI
100 1 $aLUIZ, D. de B.
245 $aEvaluation of the effectiveness of ozone as a sanitizer for fish experimentally contaminated with Salmonella sp.$h[electronic resource]
260 $c2017
520 $aSalmonellosis is a major public health problem related to food contamination and ensuing food poisoning. Brazilian resolution RDC nº 12/2001 of the Brazilian National Health Surveillance Agency (ANVISA) established the absence of Salmonella in 25 g of fish for consumption. However, the significant increase in the occurrence of fish contamination by Salmonella and other pathogenic bacteria shows that the currently applied strategies are not sufficient and that, in addition to the implementation of good health practices, the application of new sanitizer technologies in the fish industry is also necessary. In this context, the present study evaluated the effectiveness of ozone in an aqueous medium as a sanitizer for Salmonella contaminated fish. The experiment was carried out using a completely randomized design with eight treatments and five replicates, giving a total of 40 experimental units. Each sample consisted of three fishes, totalizing 120 fishes. The treatments consisted of different combinations of temperature and water-dissolved ozone (O3) concentrations (21 °C × 0.35 ppm; 20 °C × 0.45 ppm; 21 °C × 0.60 ppm; 20 °C × 0.80 ppm; 19 °C × 1.7 ppm; 6 × 5.1 ppm; 4 °C × 7.2 ppm; and 2 °C × 9.1 ppm). Colossoma macropomum (Tambaqui) samples were experimentally infected with Salmonella typhymurium (ATCC 14028) and immersed in water with the different treatments. After three minutes, the fish samples were collected and subjected to qualitative Salmonella analyses. The ozone tests were not efficient in eradicating Salmonella under the experimental conditions presented here, indicating the need for the identification of effective sanitizers in order to meet the determinations of Brazilian law.
650 $aColossoma macropomum
650 $aMicrobiologia
650 $aOzônio
650 $aPeixe de água doce
650 $aSalmonella
650 $aTambaqui
700 1 $aSILVA, C. D. F. e
700 1 $aCAMPELO, S. R.
700 1 $aSANTOS, V. R. V. dos
700 1 $aLIMA, L. K. F. de
700 1 $aCHICRALA, P. C. M. S.
700 1 $aIWASHITA, M. K. P.
773 $tBrazilian Journal of Food Technology, Campinas$gv. 20, e2016150, 2017.
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Registro original: |
Embrapa Pesca e Aquicultura (CNPASA) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Trigo. Para informações adicionais entre em contato com cnpt.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia; Embrapa Trigo. |
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Data corrente: |
21/02/2022 |
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Data da última atualização: |
05/09/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
ROESLER, E. A.; BALBINOTT, N.; SCHROEDER, R. G.; LAU, E. Y.; LIMA, M. I. P. M.; BRAMMER, S. P.; ARAGÃO, F. J. L.; FERNANDES, J. M. C.; DEUNER, C. C. |
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Afiliação: |
EDUARDO ANDRÉ ROESLER, UPF; NATALIA BALBINOTT, UFRGS; RENATA GABRIELA SCHROEDER, UPF; ELENE YAMAZAKI LAU, CNPT; MARIA IMACULADA PONTES M LIMA, CNPT; SANDRA PATUSSI BRAMMER, CNPT; FRANCISCO JOSE LIMA ARAGAO, Cenargen; JOSE MAURICIO CUNHA FERNANDES, CNPT; CAROLINA CARDOSO DEUNER, UPF. |
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Título: |
An efficient protocol for inoculation of Fusarium graminearum in detached leaves of Arabidopsis. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Tropical Plant Pathology, 16 Feb. 2022 |
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DOI: |
https://doi.org/10.1007/s40858-022-00497-x |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Fusarium graminearum is the main cause of Fusarium head blight, a fungal disease that reduces yield and afects the quality of wheat and other small grains. Genetic resistance is the ideal method for disease control; however, there are not yet cultivars with sufcient resistance levels to withstand an epidemic. Genetic engineering strategies such as gene silencing, host-induced gene silencing (HIGS), overexpression, and genome editing are promising, but many target genes need to be analyzed to fnd a suitable one. Arabidopsis thaliana is a model plant that is also susceptible to F. graminearum. Although this interaction was reported nearly two decades ago, consistent infection and symptoms are not always obtained. The availability of an efcient inoculation method is essential for studying plant-pathogen interaction. This work aimed at testing protocols for inoculating F. graminearum in detached leaves of A. thaliana which varied in inoculation site (leaf sides), wound (abrasion or hole injury), and inoculum type (mycelium agar disk or spore). We found that a mycelium agar disk placed on an abrasion injury at the adaxial leaf side was the most efcient (highest lesion size, incidence, and easiness to visualize the symptoms caused by the pathogen) method to inoculate F. graminearum in detached leaves of Arabidopsis. |
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Palavras-Chave: |
Inoculation assay; Protocol. |
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Thesagro: |
Doença Fúngica; Ferrugem; Fusarium Graminearum; Trigo. |
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Thesaurus NAL: |
Arabidopsis thaliana; Fusarium head blight. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02292naa a2200325 a 4500
001 2140281
005 2022-09-05
008 2022 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s40858-022-00497-x$2DOI
100 1 $aROESLER, E. A.
245 $aAn efficient protocol for inoculation of Fusarium graminearum in detached leaves of Arabidopsis.$h[electronic resource]
260 $c2022
520 $aAbstract: Fusarium graminearum is the main cause of Fusarium head blight, a fungal disease that reduces yield and afects the quality of wheat and other small grains. Genetic resistance is the ideal method for disease control; however, there are not yet cultivars with sufcient resistance levels to withstand an epidemic. Genetic engineering strategies such as gene silencing, host-induced gene silencing (HIGS), overexpression, and genome editing are promising, but many target genes need to be analyzed to fnd a suitable one. Arabidopsis thaliana is a model plant that is also susceptible to F. graminearum. Although this interaction was reported nearly two decades ago, consistent infection and symptoms are not always obtained. The availability of an efcient inoculation method is essential for studying plant-pathogen interaction. This work aimed at testing protocols for inoculating F. graminearum in detached leaves of A. thaliana which varied in inoculation site (leaf sides), wound (abrasion or hole injury), and inoculum type (mycelium agar disk or spore). We found that a mycelium agar disk placed on an abrasion injury at the adaxial leaf side was the most efcient (highest lesion size, incidence, and easiness to visualize the symptoms caused by the pathogen) method to inoculate F. graminearum in detached leaves of Arabidopsis.
650 $aArabidopsis thaliana
650 $aFusarium head blight
650 $aDoença Fúngica
650 $aFerrugem
650 $aFusarium Graminearum
650 $aTrigo
653 $aInoculation assay
653 $aProtocol
700 1 $aBALBINOTT, N.
700 1 $aSCHROEDER, R. G.
700 1 $aLAU, E. Y.
700 1 $aLIMA, M. I. P. M.
700 1 $aBRAMMER, S. P.
700 1 $aARAGÃO, F. J. L.
700 1 $aFERNANDES, J. M. C.
700 1 $aDEUNER, C. C.
773 $tTropical Plant Pathology, 16 Feb. 2022
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Embrapa Trigo (CNPT) |
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