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 | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
18/06/2012 |
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Data da última atualização: |
21/01/2025 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CAVALCANTE, A. C. R.; FERREIRA, A. M.; MELO, M. N.; FUX, B.; BRANDÃO, G. P.; VITOR, R. W. A. |
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Afiliação: |
ANTONIO CEZAR ROCHA CAVALCANTE, CNPC; A.M. Ferreira. |
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Título: |
Virulence and molecular characterization of Toxoplasma gondii isolated from goats in Ceará, Brazil. |
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Ano de publicação: |
2007 |
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Fonte/Imprenta: |
Small Ruminant Research, v. 69, n. 1/3, p. 79-82, May, 2007. |
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DOI: |
10.1016/j.smallrumres.2005.12.023 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract - One hundred and sixty-nine fragments of heart muscle collected from goats in the State of Cear´ a, Brazil, were analyzed by mouse bioassay with the aim of isolating Toxoplasma gondii.Two T. gondii isolates, named G1 and G2, were obtained and were characterized by PCR-RFLP. In addition, their virulence was evaluated by experimental inoculation into BALB/c mice. The G1 isolate presented high virulence leading to 100% mortality of mice after inoculations with 101, 102, and 103 tachyzoites. The G2 isolate presented low virulence and none of the doses tested lead to mortality of mice. The PCR-RFLP analysis showed that the two isolates are recombinants of the types I/III. However, they differ in the haplotype combination for the genotypes analyzed. |
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Palavras-Chave: |
PCR-RFLP. |
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Thesagro: |
Caprino; Toxoplasma Gondii. |
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Thesaurus Nal: |
Brazil; Goats; Virulence. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 01535naa a2200265 a 4500
001 1926597
005 2025-01-21
008 2007 bl uuuu u00u1 u #d
024 7 $a10.1016/j.smallrumres.2005.12.023$2DOI
100 1 $aCAVALCANTE, A. C. R.
245 $aVirulence and molecular characterization of Toxoplasma gondii isolated from goats in Ceará, Brazil.$h[electronic resource]
260 $c2007
520 $aAbstract - One hundred and sixty-nine fragments of heart muscle collected from goats in the State of Cear´ a, Brazil, were analyzed by mouse bioassay with the aim of isolating Toxoplasma gondii.Two T. gondii isolates, named G1 and G2, were obtained and were characterized by PCR-RFLP. In addition, their virulence was evaluated by experimental inoculation into BALB/c mice. The G1 isolate presented high virulence leading to 100% mortality of mice after inoculations with 101, 102, and 103 tachyzoites. The G2 isolate presented low virulence and none of the doses tested lead to mortality of mice. The PCR-RFLP analysis showed that the two isolates are recombinants of the types I/III. However, they differ in the haplotype combination for the genotypes analyzed.
650 $aBrazil
650 $aGoats
650 $aVirulence
650 $aCaprino
650 $aToxoplasma Gondii
653 $aPCR-RFLP
700 1 $aFERREIRA, A. M.
700 1 $aMELO, M. N.
700 1 $aFUX, B.
700 1 $aBRANDÃO, G. P.
700 1 $aVITOR, R. W. A.
773 $tSmall Ruminant Research$gv. 69, n. 1/3, p. 79-82, May, 2007.
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