01523naa a2200253 a 450000100080000000500110000800800410001902400430006010000250010324501280012826000090025652007690026565000100103465000140104465000120105865000220107065300130109270000200110570000160112570000120114170000200115370000200117377300760119319265972014-09-15       2007    bl uuuu     u00u1 u    #d7 a10.1016/j.smallrumres.2005.12.0232DOI1 aCAVALCANTE, A. C. R.  aVirulence and molecular characterization of Toxoplasma gondii isolated from goats in Ceará, Brazil.h[electronic resource]  c2007  aAbstract - One hundred and sixty-nine fragments of heart muscle collected from goats in the State of Cear´ a, Brazil, were analyzed by mouse bioassay with the aim of isolating Toxoplasma gondii.Two T. gondii isolates, named G1 and G2, were obtained and were characterized by PCR-RFLP. In addition, their virulence was evaluated by experimental inoculation into BALB/c mice. The G1 isolate presented high virulence leading to 100% mortality of mice after inoculations with 101, 102, and 103 tachyzoites. The G2 isolate presented low virulence and none of the doses tested lead to mortality of mice. The PCR-RFLP analysis showed that the two isolates are recombinants of the types I/III. However, they differ in the haplotype combination for the genotypes analyzed.  aGoats  aVirulence  aCaprino  aToxoplasma Gondii  aPCR-RFLP1 aFERREIRA, A. M.1 aMELO, M. N.1 aFUX, B.1 aBRANDÃO, G. P.1 aVITOR, R. W. A.  tSmall Ruminant Research, Amsterdamgv. 69, n. 1/3, p. 79-82, May, 2007.