01535naa a2200265 a 450000100080000000500110000800800410001902400430006010000250010324501280012826000090025652007690026565000110103465000100104565000140105565000120106965000220108165300130110370000200111670000160113670000120115270000200116470000200118477300650120419265972025-01-21       2007    bl uuuu     u00u1 u    #d7 a10.1016/j.smallrumres.2005.12.0232DOI1 aCAVALCANTE, A. C. R.  aVirulence and molecular characterization of Toxoplasma gondii isolated from goats in Ceará, Brazil.h[electronic resource]  c2007  aAbstract - One hundred and sixty-nine fragments of heart muscle collected from goats in the State of Cear´ a, Brazil, were analyzed by mouse bioassay with the aim of isolating Toxoplasma gondii.Two T. gondii isolates, named G1 and G2, were obtained and were characterized by PCR-RFLP. In addition, their virulence was evaluated by experimental inoculation into BALB/c mice. The G1 isolate presented high virulence leading to 100% mortality of mice after inoculations with 101, 102, and 103 tachyzoites. The G2 isolate presented low virulence and none of the doses tested lead to mortality of mice. The PCR-RFLP analysis showed that the two isolates are recombinants of the types I/III. However, they differ in the haplotype combination for the genotypes analyzed.  aBrazil  aGoats  aVirulence  aCaprino  aToxoplasma Gondii  aPCR-RFLP1 aFERREIRA, A. M.1 aMELO, M. N.1 aFUX, B.1 aBRANDÃO, G. P.1 aVITOR, R. W. A.  tSmall Ruminant Researchgv. 69, n. 1/3, p. 79-82, May, 2007.