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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
28/08/1995 |
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Data da última atualização: |
17/05/2023 |
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Autoria: |
KOBAYASHI, S.; IKEDA, I.; UCHIMIYA, H. |
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Afiliação: |
SHOZO KOBAYASHI, University of Tsukuba; ISAMI IKEDA; HIROFUMI UCHIMIYA. |
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Título: |
Conditions for high frequency embryogenesis from orange (Citrus sinensis Osb.) protoplasts. |
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Ano de publicação: |
1985 |
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Fonte/Imprenta: |
Plant Cell Tissue Organ Culture. v.4, p.249-259, 1985. |
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Idioma: |
Inglês |
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Conteúdo: |
Using `Trovita' orange (Citrus sinensis Osb.) protoplasts isolated from 6-year-old nucellar callus, the effects of protoplast density and mannitol concentration on cell divisions and embryoid formation were examined. Somatic embryogenesis in nearly direct manner was observed only at a combination of low cell densities (~4 x 10sobre4/ml) and low mannitol concentrations (~ 0.4 M). Two altenatives to achieve high frequency embryogenesis (~ 70%) were to either dilute the cells to lower densities, or to do serial transfers of cells to fresh medium. Orange protoplasts (cells) showed embryogenic potential, and repression of embryo-genesis occurred when propoplasts were cultured at a high density and/or under high osmotic pressure. |
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Palavras-Chave: |
Citrus sinensis Osb; Inhibitor (s); Nucellar callus; plant regeneration; Protoplast. |
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Thesaurus Nal: |
somatic embryogenesis. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01379naa a2200217 a 4500
001 1646877
005 2023-05-17
008 1985 bl uuuu u00u1 u #d
100 1 $aKOBAYASHI, S.
245 $aConditions for high frequency embryogenesis from orange (Citrus sinensis Osb.) protoplasts.$h[electronic resource]
260 $c1985
520 $aUsing `Trovita' orange (Citrus sinensis Osb.) protoplasts isolated from 6-year-old nucellar callus, the effects of protoplast density and mannitol concentration on cell divisions and embryoid formation were examined. Somatic embryogenesis in nearly direct manner was observed only at a combination of low cell densities (~4 x 10sobre4/ml) and low mannitol concentrations (~ 0.4 M). Two altenatives to achieve high frequency embryogenesis (~ 70%) were to either dilute the cells to lower densities, or to do serial transfers of cells to fresh medium. Orange protoplasts (cells) showed embryogenic potential, and repression of embryo-genesis occurred when propoplasts were cultured at a high density and/or under high osmotic pressure.
650 $asomatic embryogenesis
653 $aCitrus sinensis Osb
653 $aInhibitor (s)
653 $aNucellar callus
653 $aplant regeneration
653 $aProtoplast
700 1 $aIKEDA, I.
700 1 $aUCHIMIYA, H.
773 $tPlant Cell Tissue Organ Culture.$gv.4, p.249-259, 1985.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
