01379naa a2200217 a 450000100080000000500110000800800410001910000180006024501190007826000090019752007380020665000260094465300240097065300180099465300200101265300230103265300150105570000140107070000170108477300600110116468772023-05-17       1985    bl uuuu     u00u1 u    #d1 aKOBAYASHI, S.  aConditions for high frequency embryogenesis from orange (Citrus sinensis Osb.) protoplasts.h[electronic resource]  c1985  aUsing `Trovita' orange (Citrus sinensis Osb.) protoplasts isolated from 6-year-old nucellar callus, the effects of protoplast density and mannitol concentration on cell divisions and embryoid formation were examined. Somatic embryogenesis in nearly direct manner was observed only at a combination of low cell densities (~4 x 10sobre4/ml) and low mannitol concentrations (~ 0.4 M). Two altenatives to achieve high frequency embryogenesis (~ 70%) were to either dilute the cells to lower densities, or to do serial transfers of cells to fresh medium. Orange protoplasts (cells) showed embryogenic potential, and repression of embryo-genesis occurred when propoplasts were cultured at a high density and/or under high osmotic pressure.  asomatic embryogenesis  aCitrus sinensis Osb  aInhibitor (s)  aNucellar callus  aplant regeneration  aProtoplast1 aIKEDA, I.1 aUCHIMIYA, H.  tPlant Cell Tissue Organ Culture.gv.4, p.249-259, 1985.