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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
19/11/2003 |
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Data da última atualização: |
28/08/2023 |
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Autoria: |
WULSTER-RADCLIFFE, M. C.; COSTINE, B. A.; LEWIS, G. S. |
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Título: |
Estradiol-17 beta-oxytocin-induced cervical dilation in sheep: application to transcervical embryo transfer. |
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Ano de publicação: |
1999 |
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Fonte/Imprenta: |
Journal of Animal Science, v. 77, n. 10, p. 2587-2593, Oct. 1999. |
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DOI: |
10.2527/1999.77102587x. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Experiments were conducted to determine whether exogenous estradiol-17beta (E2) and oxytocin (OT) can be used to improve transcervical (TC) embryo transfer (ET) procedures for sheep. Our concerns that the E2-OT treatment may alter luteal function prompted Exp. 1, in which 32 ewes were assigned to treatments in a 2x2 factorial array. On d 7 after onset of estrus, ewes received i.v. either 100 microg of E2 or diluent; 12 h later, ewes received i.v. either 400 USP units of OT or saline. To monitor luteal function, progesterone was measured in jugular blood collected from d 7 to 18. The treatments did not affect progesterone concentrations. Two trials were conducted in Exp. 2. In Trial 1, ewes were assigned to one of three treatments: TC transfer with E2-OT treatment to dilate the cervix, laparoscopic ET with E2-OT treatment, or laparoscopic ET with an equivalent diluent that did not dilate the cervix. In Trial 2, ewes were assigned to treatments in a 2x2 factorial array: TC or laparoscopic ET on d 6; E2-OT treatment for cervical dilation or diluents on d 6. Transferred embryos were recovered on d 12 in Trial 1 and d 14 in Trial 2, evaluated morphologically for development, and scored. Treatments did not affect the percentage of transferred embryos recovered. However, mode of transfer decreased (P<.01) the mean embryo development score. The E2-OT treatment increased (P<.01) the development score of embryos transferred transcervically, indicating that cervical dilation may improve the chances of embryos surviving after TC transfer. In conclusion, E2-OT treatment did not affect luteal function, and the E2-OT treatment can be used to enhance the success of TC embryo transfer in sheep. MenosAbstract: Experiments were conducted to determine whether exogenous estradiol-17beta (E2) and oxytocin (OT) can be used to improve transcervical (TC) embryo transfer (ET) procedures for sheep. Our concerns that the E2-OT treatment may alter luteal function prompted Exp. 1, in which 32 ewes were assigned to treatments in a 2x2 factorial array. On d 7 after onset of estrus, ewes received i.v. either 100 microg of E2 or diluent; 12 h later, ewes received i.v. either 400 USP units of OT or saline. To monitor luteal function, progesterone was measured in jugular blood collected from d 7 to 18. The treatments did not affect progesterone concentrations. Two trials were conducted in Exp. 2. In Trial 1, ewes were assigned to one of three treatments: TC transfer with E2-OT treatment to dilate the cervix, laparoscopic ET with E2-OT treatment, or laparoscopic ET with an equivalent diluent that did not dilate the cervix. In Trial 2, ewes were assigned to treatments in a 2x2 factorial array: TC or laparoscopic ET on d 6; E2-OT treatment for cervical dilation or diluents on d 6. Transferred embryos were recovered on d 12 in Trial 1 and d 14 in Trial 2, evaluated morphologically for development, and scored. Treatments did not affect the percentage of transferred embryos recovered. However, mode of transfer decreased (P<.01) the mean embryo development score. The E2-OT treatment increased (P<.01) the development score of embryos transferred transcervically, indicating that cervical dilation ... Mostrar Tudo |
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Palavras-Chave: |
Cervix Uteri; Drug effects; Ocitocina. |
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Thesagro: |
Endocrinologia; Estradiol; Hormônio; Ovino; Reprodução Animal; Transferência de Embrião. |
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Thesaurus Nal: |
Embryo transfer; Estrus; Laparotomy; Oxytocin; Pharmacology; Sheep. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02682naa a2200337 a 4500
001 1530040
005 2023-08-28
008 1999 bl uuuu u00u1 u #d
024 7 $a10.2527/1999.77102587x.$2DOI
100 1 $aWULSTER-RADCLIFFE, M. C.
245 $aEstradiol-17 beta-oxytocin-induced cervical dilation in sheep$bapplication to transcervical embryo transfer.$h[electronic resource]
260 $c1999
520 $aAbstract: Experiments were conducted to determine whether exogenous estradiol-17beta (E2) and oxytocin (OT) can be used to improve transcervical (TC) embryo transfer (ET) procedures for sheep. Our concerns that the E2-OT treatment may alter luteal function prompted Exp. 1, in which 32 ewes were assigned to treatments in a 2x2 factorial array. On d 7 after onset of estrus, ewes received i.v. either 100 microg of E2 or diluent; 12 h later, ewes received i.v. either 400 USP units of OT or saline. To monitor luteal function, progesterone was measured in jugular blood collected from d 7 to 18. The treatments did not affect progesterone concentrations. Two trials were conducted in Exp. 2. In Trial 1, ewes were assigned to one of three treatments: TC transfer with E2-OT treatment to dilate the cervix, laparoscopic ET with E2-OT treatment, or laparoscopic ET with an equivalent diluent that did not dilate the cervix. In Trial 2, ewes were assigned to treatments in a 2x2 factorial array: TC or laparoscopic ET on d 6; E2-OT treatment for cervical dilation or diluents on d 6. Transferred embryos were recovered on d 12 in Trial 1 and d 14 in Trial 2, evaluated morphologically for development, and scored. Treatments did not affect the percentage of transferred embryos recovered. However, mode of transfer decreased (P<.01) the mean embryo development score. The E2-OT treatment increased (P<.01) the development score of embryos transferred transcervically, indicating that cervical dilation may improve the chances of embryos surviving after TC transfer. In conclusion, E2-OT treatment did not affect luteal function, and the E2-OT treatment can be used to enhance the success of TC embryo transfer in sheep.
650 $aEmbryo transfer
650 $aEstrus
650 $aLaparotomy
650 $aOxytocin
650 $aPharmacology
650 $aSheep
650 $aEndocrinologia
650 $aEstradiol
650 $aHormônio
650 $aOvino
650 $aReprodução Animal
650 $aTransferência de Embrião
653 $aCervix Uteri
653 $aDrug effects
653 $aOcitocina
700 1 $aCOSTINE, B. A.
700 1 $aLEWIS, G. S.
773 $tJournal of Animal Science$gv. 77, n. 10, p. 2587-2593, Oct. 1999.
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Embrapa Caprinos e Ovinos (CNPC) |
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