02682naa a2200337 a 450000100080000000500110000800800410001902400330006010000290009324501360012226000090025852017250026765000200199265000110201265000150202365000130203865000170205165000100206865000190207865000140209765000140211165000100212565000240213565000310215965300170219065300170220765300140222470000190223870000170225777300700227415300402023-08-28       1999    bl uuuu     u00u1 u    #d7 a10.2527/1999.77102587x.2DOI1 aWULSTER-RADCLIFFE, M. C.  aEstradiol-17 beta-oxytocin-induced cervical dilation in sheepbapplication to transcervical embryo transfer.h[electronic resource]  c1999  aAbstract: Experiments were conducted to determine whether exogenous estradiol-17beta (E2) and oxytocin (OT) can be used to improve transcervical (TC) embryo transfer (ET) procedures for sheep. Our concerns that the E2-OT treatment may alter luteal function prompted Exp. 1, in which 32 ewes were assigned to treatments in a 2x2 factorial array. On d 7 after onset of estrus, ewes received i.v. either 100 microg of E2 or diluent; 12 h later, ewes received i.v. either 400 USP units of OT or saline. To monitor luteal function, progesterone was measured in jugular blood collected from d 7 to 18. The treatments did not affect progesterone concentrations. Two trials were conducted in Exp. 2. In Trial 1, ewes were assigned to one of three treatments: TC transfer with E2-OT treatment to dilate the cervix, laparoscopic ET with E2-OT treatment, or laparoscopic ET with an equivalent diluent that did not dilate the cervix. In Trial 2, ewes were assigned to treatments in a 2x2 factorial array: TC or laparoscopic ET on d 6; E2-OT treatment for cervical dilation or diluents on d 6. Transferred embryos were recovered on d 12 in Trial 1 and d 14 in Trial 2, evaluated morphologically for development, and scored. Treatments did not affect the percentage of transferred embryos recovered. However, mode of transfer decreased (P&lt.01) the mean embryo development score. The E2-OT treatment increased (P&lt.01) the development score of embryos transferred transcervically, indicating that cervical dilation may improve the chances of embryos surviving after TC transfer. In conclusion, E2-OT treatment did not affect luteal function, and the E2-OT treatment can be used to enhance the success of TC embryo transfer in sheep.  aEmbryo transfer  aEstrus  aLaparotomy  aOxytocin  aPharmacology  aSheep  aEndocrinologia  aEstradiol  aHormônio  aOvino  aReprodução Animal  aTransferência de Embrião  aCervix Uteri  aDrug effects  aOcitocina1 aCOSTINE, B. A.1 aLEWIS, G. S.  tJournal of Animal Sciencegv. 77, n. 10, p. 2587-2593, Oct. 1999.