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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
20/02/1997 |
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Data da última atualização: |
28/06/2023 |
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Autoria: |
UPREDI, G. C.; PAYNE, S. R.; DUGANZICH, D. M.; OLIVER, J. E.; SMITH, J. F. |
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Título: |
Enzyme leakage during cryopreservation of ram spermatozoa. |
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Ano de publicação: |
1996 |
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Fonte/Imprenta: |
Animal Reproduction Science, v. 41, n. 1, p. 27-36, Jan. 1996. |
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DOI: |
https://doi.org/10.1016/0378-4320(95)01442-X |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Spermatozoal motility and enzyme leakage during cryopreservation of ram spermatozoa was evaluated in the presence and absence of antifreeze proteins (AFP). Loss of spermatozoal motility due to the cryopreservation process was reduced by 10% in the presence of AFP. Samples of the diluted semen at various stages of the cryopreservation process were assayed for aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP), acid phosphatase (ACP) and lactate dehydrogenase (LDH). The levels of AST, ALT and ACP were low in ram spermatozoa and were not considered suitable for the objective measurement of spermatozoal damage. ALP leaked during the cooling and freezing process, whereas LDH leakage was prevalent during cooling, freezing and post-thaw incubation of spermatozoa. Changes in ALP and LDH could be used as marker enzymes in the development of semen processing protocols and of semen diluents. |
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Thesagro: |
Criopreservação; Enzima; Espermatozóide; Ovino; Sêmen. |
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Thesaurus Nal: |
Alkaline phosphatase; Antifreeze proteins; Cold storage; Males; Rams; Reproduction; Sheep; Spermatozoa. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 01849naa a2200337 a 4500
001 1523457
005 2023-06-28
008 1996 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/0378-4320(95)01442-X$2DOI
100 1 $aUPREDI, G. C.
245 $aEnzyme leakage during cryopreservation of ram spermatozoa.$h[electronic resource]
260 $c1996
520 $aAbstract: Spermatozoal motility and enzyme leakage during cryopreservation of ram spermatozoa was evaluated in the presence and absence of antifreeze proteins (AFP). Loss of spermatozoal motility due to the cryopreservation process was reduced by 10% in the presence of AFP. Samples of the diluted semen at various stages of the cryopreservation process were assayed for aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP), acid phosphatase (ACP) and lactate dehydrogenase (LDH). The levels of AST, ALT and ACP were low in ram spermatozoa and were not considered suitable for the objective measurement of spermatozoal damage. ALP leaked during the cooling and freezing process, whereas LDH leakage was prevalent during cooling, freezing and post-thaw incubation of spermatozoa. Changes in ALP and LDH could be used as marker enzymes in the development of semen processing protocols and of semen diluents.
650 $aAlkaline phosphatase
650 $aAntifreeze proteins
650 $aCold storage
650 $aMales
650 $aRams
650 $aReproduction
650 $aSheep
650 $aSpermatozoa
650 $aCriopreservação
650 $aEnzima
650 $aEspermatozóide
650 $aOvino
650 $aSêmen
700 1 $aPAYNE, S. R.
700 1 $aDUGANZICH, D. M.
700 1 $aOLIVER, J. E.
700 1 $aSMITH, J. F.
773 $tAnimal Reproduction Science$gv. 41, n. 1, p. 27-36, Jan. 1996.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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