01849naa a2200337 a 450000100080000000500110000800800410001902400540006010000180011424500860013226000090021852009420022765000250116965000240119465000170121865000100123565000090124565000170125465000100127165000160128165000220129765000110131965000200133065000100135065000110136070000170137170000210138870000180140970000170142777300670144415234572023-06-28       1996    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.1016/0378-4320(95)01442-X2DOI1 aUPREDI, G. C.  aEnzyme leakage during cryopreservation of ram spermatozoa.h[electronic resource]  c1996  aAbstract: Spermatozoal motility and enzyme leakage during cryopreservation of ram spermatozoa was evaluated in the presence and absence of antifreeze proteins (AFP). Loss of spermatozoal motility due to the cryopreservation process was reduced by 10% in the presence of AFP. Samples of the diluted semen at various stages of the cryopreservation process were assayed for aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP), acid phosphatase (ACP) and lactate dehydrogenase (LDH). The levels of AST, ALT and ACP were low in ram spermatozoa and were not considered suitable for the objective measurement of spermatozoal damage. ALP leaked during the cooling and freezing process, whereas LDH leakage was prevalent during cooling, freezing and post-thaw incubation of spermatozoa. Changes in ALP and LDH could be used as marker enzymes in the development of semen processing protocols and of semen diluents.  aAlkaline phosphatase  aAntifreeze proteins  aCold storage  aMales  aRams  aReproduction  aSheep  aSpermatozoa  aCriopreservação  aEnzima  aEspermatozóide  aOvino  aSêmen1 aPAYNE, S. R.1 aDUGANZICH, D. M.1 aOLIVER, J. E.1 aSMITH, J. F.  tAnimal Reproduction Sciencegv. 41, n. 1, p. 27-36, Jan. 1996.