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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
25/03/1996 |
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Data da última atualização: |
09/05/2023 |
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Autoria: |
KWANG, J.; KEEN, J.; CUTLIP, R. C.; LITTLEDIKE, E. T. |
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Título: |
Evaluation of an Elisa for detection of ovine progressive pneumonia antibodies using a recombinant transmembrane envelope protein. |
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Ano de publicação: |
1993 |
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Fonte/Imprenta: |
Journal of Veterinary Diagnostic Investigation, v. 5, n. 2, p. 189-193, 1993. |
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DOI: |
https://doi.org/10.1177/104063879300500208 |
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Idioma: |
Inglês |
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Conteúdo: |
An enzyme-linked immunosorbent assay (ELISA) was developed using a recombinant protein corresponding to the N'-terminal hydrophilic region of transmembrance glycoprotein (TM) of ovine lentivirus. This assay reproducibly detected antibodies in sera from 207 of 212 ovine progressive pneumonia (OPP) virus-infected sheep, and the recombinant TM ELISA accurately identified 26% (35 vs.9) more seropositive samples than did the agar gel immunodiffusion test when applied to 100 sera from an infected flock. This assay also yielded no false-positive results in 14 true negative sera. Results of these experiments were further confirmed by the recombinant TM and recombinant p25 Western blot assay. A single recombinant TM antigen, as the coating antigen in ELISA, can be usedsucessfully for the detection of OPP virus-infected animals and can improve the sensitivity and specificity for OPP diagnosis. |
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Thesagro: |
Doença; Elisa; Ovino; Pneumonia. |
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Thesaurus Nal: |
Immunoblotting; Ovine lentivirus; Sheep diseases; Viral pneumonia. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 01720naa a2200265 a 4500
001 1523406
005 2023-05-09
008 1993 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1177/104063879300500208$2DOI
100 1 $aKWANG, J.
245 $aEvaluation of an Elisa for detection of ovine progressive pneumonia antibodies using a recombinant transmembrane envelope protein.$h[electronic resource]
260 $c1993
520 $aAn enzyme-linked immunosorbent assay (ELISA) was developed using a recombinant protein corresponding to the N'-terminal hydrophilic region of transmembrance glycoprotein (TM) of ovine lentivirus. This assay reproducibly detected antibodies in sera from 207 of 212 ovine progressive pneumonia (OPP) virus-infected sheep, and the recombinant TM ELISA accurately identified 26% (35 vs.9) more seropositive samples than did the agar gel immunodiffusion test when applied to 100 sera from an infected flock. This assay also yielded no false-positive results in 14 true negative sera. Results of these experiments were further confirmed by the recombinant TM and recombinant p25 Western blot assay. A single recombinant TM antigen, as the coating antigen in ELISA, can be usedsucessfully for the detection of OPP virus-infected animals and can improve the sensitivity and specificity for OPP diagnosis.
650 $aImmunoblotting
650 $aOvine lentivirus
650 $aSheep diseases
650 $aViral pneumonia
650 $aDoença
650 $aElisa
650 $aOvino
650 $aPneumonia
700 1 $aKEEN, J.
700 1 $aCUTLIP, R. C.
700 1 $aLITTLEDIKE, E. T.
773 $tJournal of Veterinary Diagnostic Investigation$gv. 5, n. 2, p. 189-193, 1993.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
