01720naa a2200265 a 450000100080000000500110000800800410001902400520006010000140011224501580012626000090028452009010029365000190119465000210121365000190123465000200125365000120127365000100128565000100129565000140130570000130131970000180133270000220135077300820137215234062023-05-09       1993    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.1177/1040638793005002082DOI1 aKWANG, J.  aEvaluation of an Elisa for detection of ovine progressive pneumonia antibodies using a recombinant transmembrane envelope protein.h[electronic resource]  c1993  aAn enzyme-linked immunosorbent assay (ELISA) was developed using a recombinant protein corresponding to the N'-terminal hydrophilic region of transmembrance glycoprotein (TM) of ovine lentivirus. This assay reproducibly detected antibodies in sera from 207 of 212 ovine progressive pneumonia (OPP) virus-infected sheep, and the recombinant TM ELISA accurately identified 26% (35 vs.9) more seropositive samples than did the agar gel immunodiffusion test when applied to 100 sera from an infected flock. This assay also yielded no false-positive results in 14 true negative sera. Results of these experiments were further confirmed by the recombinant TM and recombinant p25 Western blot assay. A single recombinant TM antigen, as the coating antigen in ELISA, can be usedsucessfully for the detection of OPP virus-infected animals and can improve the sensitivity and specificity for OPP diagnosis.  aImmunoblotting  aOvine lentivirus  aSheep diseases  aViral pneumonia  aDoença  aElisa  aOvino  aPneumonia1 aKEEN, J.1 aCUTLIP, R. C.1 aLITTLEDIKE, E. T.  tJournal of Veterinary Diagnostic Investigationgv. 5, n. 2, p. 189-193, 1993.