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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agricultura Digital. Para informações adicionais entre em contato com cnptia.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Agricultura Digital. |
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Data corrente: |
26/03/2009 |
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Data da última atualização: |
15/01/2020 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
FALCAO, P. R. K.; TEIXEIRA, K. R. dos. |
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Afiliação: |
PAULA REGINA KUSER FALCAO, CNPTIA; KATIA REGINA DOS SANTOS TEIXEIRA, CNPAB. |
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Título: |
Evaluation of point mutation in genes related to ketogluconic acid production by Gluconacetobacter diazotrophicus. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
In: INTERNATIONAL CONFERENCE OF BRAZILIAN ASSOCIATION FOR BIOINFORMATICS AND COMPUTATIONAL BIOLOGY, 4., 2008, Salvador. Proceedings... Salvador: AB³C, 2008. |
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Páginas: |
Não paginado. |
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Idioma: |
Inglês |
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Notas: |
X-Meeting 2008. |
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Conteúdo: |
Here, bioinformatic approach based on protein modeling was applied to two ORFs related to ketogluconates production, gluconate 5-dehydrogenase and 2-ketoglucone reductase. The ORFs were analyzed and identification of regions that play an important role in substrate binding/recognition presents an opportunity for stability prediction and design of mutagenesis strategy. Gluconate-5-dehydrogenase was modelled using as a 3D model the crystal structure of Gluconate 5-dehydrogenase from Thermotoga maritima (PDB ID: vl8) whereas crystal structure of a NDA-dependent p-glycerate dehydrogenase (PDB ID:1gdh) was used to model 2-ketogluconate reductase. The sequence identities for these proteins are 36% and 34%, respectively. Based on this in silico model and with the prediction performed with CUPSAT [4], it was proposed to construct the mutants His289Asn and Glu271Ser of 2-ketoglucone reductase. |
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Palavras-Chave: |
Bioinformática; Gluconacetobacter diazotrophics; Oxidação da glicose; Produção de ácido. |
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Thesagro: |
Fixação de Nitrogênio. |
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Thesaurus Nal: |
Bioinformatics; genes. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
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001 1031806
005 2020-01-15
008 2008 bl uuuu u00u1 u #d
100 1 $aFALCAO, P. R. K.
245 $aEvaluation of point mutation in genes related to ketogluconic acid production by Gluconacetobacter diazotrophicus.$h[electronic resource]
260 $aIn: INTERNATIONAL CONFERENCE OF BRAZILIAN ASSOCIATION FOR BIOINFORMATICS AND COMPUTATIONAL BIOLOGY, 4., 2008, Salvador. Proceedings... Salvador: AB³C$c2008
300 $aNão paginado.
500 $aX-Meeting 2008.
520 $aHere, bioinformatic approach based on protein modeling was applied to two ORFs related to ketogluconates production, gluconate 5-dehydrogenase and 2-ketoglucone reductase. The ORFs were analyzed and identification of regions that play an important role in substrate binding/recognition presents an opportunity for stability prediction and design of mutagenesis strategy. Gluconate-5-dehydrogenase was modelled using as a 3D model the crystal structure of Gluconate 5-dehydrogenase from Thermotoga maritima (PDB ID: vl8) whereas crystal structure of a NDA-dependent p-glycerate dehydrogenase (PDB ID:1gdh) was used to model 2-ketogluconate reductase. The sequence identities for these proteins are 36% and 34%, respectively. Based on this in silico model and with the prediction performed with CUPSAT [4], it was proposed to construct the mutants His289Asn and Glu271Ser of 2-ketoglucone reductase.
650 $aBioinformatics
650 $agenes
650 $aFixação de Nitrogênio
653 $aBioinformática
653 $aGluconacetobacter diazotrophics
653 $aOxidação da glicose
653 $aProdução de ácido
700 1 $aTEIXEIRA, K. R. dos
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Embrapa Agricultura Digital (CNPTIA) |
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