01744nam a2200229 a 450000100080000000500110000800800410001910000210006024501420008126001610022330000190038450000200040352009020042365000190132565000100134465000290135465300200138365300360140365300260143965300250146570000240149010318062020-01-15       2008    bl uuuu     u00u1 u    #d1 aFALCAO, P. R. K.  aEvaluation of point mutation in genes related to ketogluconic acid production by Gluconacetobacter diazotrophicus.h[electronic resource]  aIn: INTERNATIONAL CONFERENCE OF BRAZILIAN ASSOCIATION FOR BIOINFORMATICS AND COMPUTATIONAL BIOLOGY, 4., 2008, Salvador. Proceedings... Salvador: AB³Cc2008  aNão paginado.  aX-Meeting 2008.  aHere, bioinformatic approach based on protein modeling was applied to two ORFs related to ketogluconates production, gluconate 5-dehydrogenase and 2-ketoglucone reductase. The ORFs were analyzed and identification of regions that play an important role in substrate binding/recognition presents an opportunity for stability prediction and design of mutagenesis strategy. Gluconate-5-dehydrogenase was modelled using as a 3D model the crystal structure of Gluconate 5-dehydrogenase from Thermotoga maritima (PDB ID: vl8) whereas crystal structure of a NDA-dependent p-glycerate dehydrogenase (PDB ID:1gdh) was used to model 2-ketogluconate reductase. The sequence identities for these proteins are 36% and 34%, respectively. Based on this in silico model and with the prediction performed with CUPSAT [4], it was proposed to construct the mutants His289Asn and Glu271Ser of 2-ketoglucone reductase.  aBioinformatics  agenes  aFixação de Nitrogênio  aBioinformática  aGluconacetobacter diazotrophics  aOxidação da glicose  aProdução de ácido1 aTEIXEIRA, K. R. dos