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Registro Completo |
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
21/06/2004 |
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Data da última atualização: |
25/11/2024 |
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Autoria: |
DOYLE, J. J.; DOYLE, J. L. |
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Título: |
A rapid DNA isolation procedure for small quantities of fresh leaf tissue. |
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Ano de publicação: |
1987 |
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Fonte/Imprenta: |
Phytochemical Bulletin, v. 19, n. 1, p. 11-15, 1987. |
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Idioma: |
Inglês |
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Conteúdo: |
Of the several rapid and inexpensive DNA isolation procedures that have been described recently, one of the most popular is that of Saghai-Maroof et al. (1984), a procedure using hexadecyltrimethylammonium bromide (CTAB) and lyophilized tissue. We here describe a simple modification of this procedure that has been found to be efficient for nucleic acid isolations from fresh leaf tissue, and which we have also used for dried herbarium specimens. |
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Palavras-Chave: |
Brometo de hexadeciltrimetilamônio; CTAB; Sistemática molecular. |
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Thesagro: |
Cultura de tecido; DNA; Isolamento. |
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Thesaurus Nal: |
Tissue culture. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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Marc: |
LEADER 01050naa a2200217 a 4500
001 1127521
005 2024-11-25
008 1987 bl --- 0-- u #d
100 1 $aDOYLE, J. J.
245 $aA rapid DNA isolation procedure for small quantities of fresh leaf tissue.
260 $c1987
520 $aOf the several rapid and inexpensive DNA isolation procedures that have been described recently, one of the most popular is that of Saghai-Maroof et al. (1984), a procedure using hexadecyltrimethylammonium bromide (CTAB) and lyophilized tissue. We here describe a simple modification of this procedure that has been found to be efficient for nucleic acid isolations from fresh leaf tissue, and which we have also used for dried herbarium specimens.
650 $aTissue culture
650 $aCultura de tecido
650 $aDNA
650 $aIsolamento
653 $aBrometo de hexadeciltrimetilamônio
653 $aCTAB
653 $aSistemática molecular
700 1 $aDOYLE, J. L.
773 $tPhytochemical Bulletin$gv. 19, n. 1, p. 11-15, 1987.
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Registro original: |
Embrapa Semiárido (CPATSA) |
