01050naa a2200217 a 450000100080000000500110000800800410001910000170006024500790007726000090015652004530016565000190061865000220063765000080065965000150066765300400068265300090072265300270073170000170075877300570077511275212024-11-25       1987    bl ---       0--  u    #d1 aDOYLE, J. J.  aA rapid DNA isolation procedure for small quantities of fresh leaf tissue.  c1987  aOf the several rapid and inexpensive DNA isolation procedures that have been described recently, one of the most popular is that of Saghai-Maroof et al. (1984), a procedure using hexadecyltrimethylammonium bromide (CTAB) and lyophilized tissue. We here describe a simple modification of this procedure that has been found to be efficient for nucleic acid isolations from fresh leaf tissue, and which we have also used for dried herbarium specimens.  aTissue culture  aCultura de tecido  aDNA  aIsolamento  aBrometo de hexadeciltrimetilamônio  aCTAB  aSistemática molecular1 aDOYLE, J. L.  tPhytochemical Bulletingv. 19, n. 1, p. 11-15, 1987.