| |
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Recursos Genéticos e Biotecnologia. Para informações adicionais entre em contato com cenargen.biblioteca@embrapa.br. |
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
|
Data corrente: |
10/06/2025 |
|
Data da última atualização: |
10/06/2025 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
GOMES, H. T.; MONTEIRO, T. R.; CAMILLO, J.; NOGUEIRA, G. F.; COSTA, F. H. da S.; SILVA-CARDOSO, I. M. de A.; PEREIRA, J. E. S. |
|
Afiliação: |
HUGO TEIXEIRA GOMES, UNIVERSITY OF BRASÍLIA; TATIANE ROSA MONTEIRO, UNIVERSITY OF BRASÍLIA; JULCEIA CAMILLO, UNIVERSITY OF BRASÍLIA; GABRIELA FERREIRA NOGUEIRA; FREDERICO HENRIQUE DA SILVA COSTA, FEDERAL UNIVERSITY OF ACRE; INAÊ MARIÊ DE ARAÚJO SILVA-CARDOSO; JONNY EVERSON SCHERWINSKI PEREIRA, CENARGEN. |
|
Título: |
Integrated seed cryopreservation, bioreactor and conventional micropropagation, and genetic stability assessment by flow cytometry of Uncaria guianensis (Aubl.) J.F.Gmel, a high-value Amazonian medicinal plant. |
|
Ano de publicação: |
2025 |
|
Fonte/Imprenta: |
Plant Cell, Tissue and Organ Culture (PCTOC), v. 161, 45, 2025. |
|
DOI: |
https://doi.org/10.1007/s11240-025-03077-4 |
|
Idioma: |
Inglês |
|
Notas: |
Na publicação: Jonny Everson Scherwinski-Pereira. |
|
Conteúdo: |
This study presents an optimized protocol for the ex-situ conservation and micropropagation of Uncaria guianensis, a medicinal plant, using conventional and bioreactor-based tissue culture systems. Seeds were desiccated (0, 24, and 48 h) and then stored at–20 ºC or–196 ºC (liquid nitrogen) for up to 365 days, with or without cryoprotectants. In vitro propagation was performed using stem segments cultured in semi-solid, liquid, and bioreactors systems (RITA® and Twin Flask– TIS®). The most efficient system was further optimized by testing MS and WPM media with 6-benzylaminopurine (BAP) (0–4.0 mg L−1) over three subcultures. Micro-cuttings were rooted in MS or WPM media supplemented with indole-3-butyric acid (IBA) (0–1.0 mg L−1). Results showed that cryopreserved seeds retained over 70% germination without desiccation or cryoprotectants. The RITA® system enhanced shoot multiplication and plant quality. WPM medium promoted greater plantlet height, while BAP (2–4 mg L−1) improved multiplication rates. Adventitious root formation reached 90%, but IBA negatively affected rooting. Acclimatized plantlets exhibited 100% survival and stable nuclear DNA content (1.85 pg). This study established an effective approach for the conservation and propagation of Uncaria guianensis. |
|
Palavras-Chave: |
Amazon; Ex-situ conservation; Plant tissue culture; Temporary immersion bioreactor. |
|
Thesaurus Nal: |
Flow cytometry; Medicinal plants. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 02371naa a2200289 a 4500
001 2176542
005 2025-06-10
008 2025 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s11240-025-03077-4$2DOI
100 1 $aGOMES, H. T.
245 $aIntegrated seed cryopreservation, bioreactor and conventional micropropagation, and genetic stability assessment by flow cytometry of Uncaria guianensis (Aubl.) J.F.Gmel, a high-value Amazonian medicinal plant.$h[electronic resource]
260 $c2025
500 $aNa publicação: Jonny Everson Scherwinski-Pereira.
520 $aThis study presents an optimized protocol for the ex-situ conservation and micropropagation of Uncaria guianensis, a medicinal plant, using conventional and bioreactor-based tissue culture systems. Seeds were desiccated (0, 24, and 48 h) and then stored at–20 ºC or–196 ºC (liquid nitrogen) for up to 365 days, with or without cryoprotectants. In vitro propagation was performed using stem segments cultured in semi-solid, liquid, and bioreactors systems (RITA® and Twin Flask– TIS®). The most efficient system was further optimized by testing MS and WPM media with 6-benzylaminopurine (BAP) (0–4.0 mg L−1) over three subcultures. Micro-cuttings were rooted in MS or WPM media supplemented with indole-3-butyric acid (IBA) (0–1.0 mg L−1). Results showed that cryopreserved seeds retained over 70% germination without desiccation or cryoprotectants. The RITA® system enhanced shoot multiplication and plant quality. WPM medium promoted greater plantlet height, while BAP (2–4 mg L−1) improved multiplication rates. Adventitious root formation reached 90%, but IBA negatively affected rooting. Acclimatized plantlets exhibited 100% survival and stable nuclear DNA content (1.85 pg). This study established an effective approach for the conservation and propagation of Uncaria guianensis.
650 $aFlow cytometry
650 $aMedicinal plants
653 $aAmazon
653 $aEx-situ conservation
653 $aPlant tissue culture
653 $aTemporary immersion bioreactor
700 1 $aMONTEIRO, T. R.
700 1 $aCAMILLO, J.
700 1 $aNOGUEIRA, G. F.
700 1 $aCOSTA, F. H. da S.
700 1 $aSILVA-CARDOSO, I. M. de A.
700 1 $aPEREIRA, J. E. S.
773 $tPlant Cell, Tissue and Organ Culture (PCTOC)$gv. 161, 45, 2025.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
| Registros recuperados : 1 | |
| 1. |  | ZALAMENA, J.; MELO, G. W. B. de; GALARZA, B. P.; BORBA, D. R.; MORAIS NETO, F. A. de; IWAMOTO, H.; ZIERO, H. D. D.; SILVA, L. S. da; BRUNETTO, G.; SCANAGATTA, V.; CAUMO, C.; ALBARELLO, J. B. Adubação da videira com plantas de cobertura. In:CONGRESSO LATINO-AMERICANO DE VITICULTURA E ENOLOGIA, 15., 2015, Bento Gonçalves, RS. p. 032. XV Congresso Latino-Americano de Viticultura e Enologia E XIII Congresso Brasileiro de Viticultura e Enologia. Bento Gonçalves-RS, 3 a 7 de Novembro de 2015.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Uva e Vinho. |
|    |
| Registros recuperados : 1 | |
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
