02371naa a2200289 a 450000100080000000500110000800800410001902400520006010000170011224502380012926000090036750000560037652013130043265000190174565000210176465300110178565300250179665300250182165300350184670000200188170000160190170000200191770000230193770000310196070000220199177300680201321765422025-06-10       2025    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.1007/s11240-025-03077-42DOI1 aGOMES, H. T.  aIntegrated seed cryopreservation, bioreactor and conventional micropropagation, and genetic stability assessment by flow cytometry of Uncaria guianensis (Aubl.) J.F.Gmel, a high-value Amazonian medicinal plant.h[electronic resource]  c2025  aNa publicação: Jonny Everson Scherwinski-Pereira.  aThis study presents an optimized protocol for the ex-situ conservation and micropropagation of Uncaria guianensis, a medicinal plant, using conventional and bioreactor-based tissue culture systems. Seeds were desiccated (0, 24, and 48 h) and then stored at–20 ºC or–196 ºC (liquid nitrogen) for up to 365 days, with or without cryoprotectants. In vitro propagation was performed using stem segments cultured in semi-solid, liquid, and bioreactors systems (RITA® and Twin Flask– TIS®). The most efficient system was further optimized by testing MS and WPM media with 6-benzylaminopurine (BAP) (0–4.0 mg L−1) over three subcultures. Micro-cuttings were rooted in MS or WPM media supplemented with indole-3-butyric acid (IBA) (0–1.0 mg L−1). Results showed that cryopreserved seeds retained over 70% germination without desiccation or cryoprotectants. The RITA® system enhanced shoot multiplication and plant quality. WPM medium promoted greater plantlet height, while BAP (2–4 mg L−1) improved multiplication rates. Adventitious root formation reached 90%, but IBA negatively affected rooting. Acclimatized plantlets exhibited 100% survival and stable nuclear DNA content (1.85 pg). This study established an effective approach for the conservation and propagation of Uncaria guianensis.  aFlow cytometry  aMedicinal plants  aAmazon  aEx-situ conservation  aPlant tissue culture  aTemporary immersion bioreactor1 aMONTEIRO, T. R.1 aCAMILLO, J.1 aNOGUEIRA, G. F.1 aCOSTA, F. H. da S.1 aSILVA-CARDOSO, I. M. de A.1 aPEREIRA, J. E. S.  tPlant Cell, Tissue and Organ Culture (PCTOC)gv. 161, 45, 2025.