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 | Acesso ao texto completo restrito à biblioteca da Embrapa Instrumentação. Para informações adicionais entre em contato com cnpdia.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Instrumentação. |
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Data corrente: |
07/05/2025 |
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Data da última atualização: |
07/05/2025 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
RAJA, S.; PASCHOALIN, R. T.; TERRA, I. A. A.; SCHALLA, C.; GUIMARÃES, F.; PERIYASAMI, G.; MATTOSO, L. H. C.; SECHI, A. |
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Afiliação: |
RWTH AACHEN UNIVERSITY; RAFAELLA TAKEHARA PASCHOALIN, UNIVERSIDADE DE SÃO PAULO (USP); IDELMA APARECIDA ALVES TERRA, UNIVERSIDADE DE SÃO PAULO (USP); RWTH AACHEN UNIVERSITY; UNIVERSITY OF SAO PAULO; KING SAUD UNIVERSITY; LUIZ HENRIQUE CAPPARELLI MATTOSO, CNPDIA; RWTH AACHEN UNIVERSITY. |
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Título: |
Highly fluorescent hybrid nanofibers as potential nanofibrous scaffolds for studying cell-fiber interactions. |
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Ano de publicação: |
2025 |
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Fonte/Imprenta: |
Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, v. 319, 124535, 2024. |
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Páginas: |
11 p. |
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ISSN: |
1386-1425 |
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Idioma: |
Português |
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Conteúdo: |
In this study, we report on the fabrication of hybrid nanofibers for labeling and bioimaging applications. Our approach is involved for developing highly fluorescent nanofibers using a blend of polylactic acid, polyethyleneglycol, and perylenediimide dyes, through the solution blow spinning technique. The nanofibers are exhibited diameters ranging from 330 nm to 420 nm. Nanofibers showed excellent red and near-infrared fluorescence emissive properties in fluorescent spectroscopy. Moreover, the strong two–photon absorption phenomenon was observed for nanofibers under confocal microscopy. To assess the applicability of these fluorescent nanofibers in bioimaging settings, we employ two types of mammalian cells B16F1 melanoma cells and J774.A1 macrophages. Both cell types exhibit negligible cytotoxicity after 24 h incubation with the nanofibers, indicating the suitability of nanofibers for cell–based experiments. We also observe strong interactions between the nanofibers and cells, as evidenced by two major events: a) the acquisition of an elongated cellular morphology. |
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Palavras-Chave: |
Cell–fiber interactions; Hybrid nanofibers; Labeling agents; Solution blow spinning. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01938naa a2200277 a 4500
001 2175354
005 2025-05-07
008 2025 bl uuuu u00u1 u #d
022 $a1386-1425
100 1 $aRAJA, S.
245 $aHighly fluorescent hybrid nanofibers as potential nanofibrous scaffolds for studying cell-fiber interactions.$h[electronic resource]
260 $c2025
300 $a11 p.
520 $aIn this study, we report on the fabrication of hybrid nanofibers for labeling and bioimaging applications. Our approach is involved for developing highly fluorescent nanofibers using a blend of polylactic acid, polyethyleneglycol, and perylenediimide dyes, through the solution blow spinning technique. The nanofibers are exhibited diameters ranging from 330 nm to 420 nm. Nanofibers showed excellent red and near-infrared fluorescence emissive properties in fluorescent spectroscopy. Moreover, the strong two–photon absorption phenomenon was observed for nanofibers under confocal microscopy. To assess the applicability of these fluorescent nanofibers in bioimaging settings, we employ two types of mammalian cells B16F1 melanoma cells and J774.A1 macrophages. Both cell types exhibit negligible cytotoxicity after 24 h incubation with the nanofibers, indicating the suitability of nanofibers for cell–based experiments. We also observe strong interactions between the nanofibers and cells, as evidenced by two major events: a) the acquisition of an elongated cellular morphology.
653 $aCell–fiber interactions
653 $aHybrid nanofibers
653 $aLabeling agents
653 $aSolution blow spinning
700 1 $aPASCHOALIN, R. T.
700 1 $aTERRA, I. A. A.
700 1 $aSCHALLA, C.
700 1 $aGUIMARÃES, F.
700 1 $aPERIYASAMI, G.
700 1 $aMATTOSO, L. H. C.
700 1 $aSECHI, A.
773 $tSpectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy$gv. 319, 124535, 2024.
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| 1. |  | SENA JUNIOR, D. G. de; PINTO, F. de A. de C.; QUEIROZ, D. de M.; MANTOVANI, E. C. Algoritmo para classificação de plantas de milho atacadas pela lagarta do cartucho (Spodoptera frugiperda, Smith) em imagens digitais. Revista Brasileira de Engenharia Agricola e Ambiental, Campina Grande, v. 5, n. 3, p. 502-509, 2001.| Biblioteca(s): Embrapa Milho e Sorgo. |
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