01938naa a2200277 a 450000100080000000500110000800800410001902200140006010000130007424501370008726000090022430000100023352010910024365300300133465300220136465300200138665300270140670000220143370000200145570000160147570000190149170000190151070000220152970000140155177300950156521753542025-05-07       2025    bl uuuu     u00u1 u    #d  a1386-14251 aRAJA, S.  aHighly fluorescent hybrid nanofibers as potential nanofibrous scaffolds for studying cell-fiber interactions.h[electronic resource]  c2025  a11 p.  aIn this study, we report on the fabrication of hybrid nanofibers for labeling and bioimaging applications. Our approach is involved for developing highly fluorescent nanofibers using a blend of polylactic acid, polyethyleneglycol, and perylenediimide dyes, through the solution blow spinning technique. The nanofibers are exhibited diameters ranging from 330 nm to 420 nm. Nanofibers showed excellent red and near-infrared fluorescence emissive properties in fluorescent spectroscopy. Moreover, the strong two–photon absorption phenomenon was observed for nanofibers under confocal microscopy. To assess the applicability of these fluorescent nanofibers in bioimaging settings, we employ two types of mammalian cells B16F1 melanoma cells and J774.A1 macrophages. Both cell types exhibit negligible cytotoxicity after 24 h incubation with the nanofibers, indicating the suitability of nanofibers for cell–based experiments. We also observe strong interactions between the nanofibers and cells, as evidenced by two major events: a) the acquisition of an elongated cellular morphology.  aCell–fiber interactions  aHybrid nanofibers  aLabeling agents  aSolution blow spinning1 aPASCHOALIN, R. T.1 aTERRA, I. A. A.1 aSCHALLA, C.1 aGUIMARÃES, F.1 aPERIYASAMI, G.1 aMATTOSO, L. H. C.1 aSECHI, A.  tSpectrochimica Acta Part A: Molecular and Biomolecular Spectroscopygv. 319, 124535, 2024.