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Registro Completo |
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Biblioteca(s): |
Embrapa Agricultura Digital. |
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Data corrente: |
11/11/2024 |
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Data da última atualização: |
11/11/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MAGALHÃES, L. O.; SOUZA, U.; RAIOL-JÚNIOR, L. L.; CARVALHO, E. V. de; MOREIRA, A. S.; FREITAS-ASTÚA, J. de; GIRARDI, E. A.; GESTEIRA, A. da S. |
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Afiliação: |
LEANDRA OLIVEIRA MAGALHÃES, UNIVERSIDADE ESTADUAL DE SANTA CRUZ; UIARA SOUZA, UNIVERSIDADE ESTADUAL DE SANTA CRUZ; LAUDECIR LEMOS RAIOL-JÚNIOR; EVERTON VIEIRA DE CARVALHO, HELIX SEMENTES E BIOTECNOLOGIA; ALECIO SOUZA MOREIRA, CNPMF; JULIANA DE FREITAS-ASTÚA, INSTITUTO BIOLÓGICO; EDUARDO AUGUSTO GIRARDI, FUNDECITRUS; ABELMON DA SILVA GESTEIRA, UNIVERSIDADE ESTADUAL DE SANTA CRUZ. |
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Título: |
Antibiotics and thermotherapy have limited effectiveness in eliminating Candidatus Liberibacter asiaticus from citrus budwood. |
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Ano de publicação: |
2024 |
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Fonte/Imprenta: |
European Journal of Plant Pathology, 2024. |
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DOI: |
https://doi.org/10.1007/s10658-024-02939-5 |
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Idioma: |
Inglês |
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Notas: |
On-line first. |
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Conteúdo: |
Abstract. Epigenetic markers related to resistance require regenerating healthy trees from previously infected plant tissues. Thus, sanitizing and regenerating healthy citrus plants from previously Candidatus Liberibacter asiaticus (CLas)-infected tissues constitute a valuable need for breeding programs. Immersion in ampicillin + streptomycin solution and thermotherapy were evaluated separately for eliminating CLas from infected citrus propagules. Valencia sweet orange (Citrus × sinensis) and trifoliate orange (Citrus trifoliata) were chosen as genotypes that were highly and moderately susceptible to CLas, respectively. Infected budwood was used to graft nucellar material with or without CLas. Thirty-two months after inoculation, 2.5-cm grafts were collected and treated by 12 h immersion in an antibiotic solution (1 g/L ampicillin + 0.1 g/L streptomycin) or in autoclaved Milli-Q water, and subsequently grafted onto Rangpur lime (C. × limonia) rootstocks. In a second experiment, thermotherapy was evaluated for treating stem cuttings kept for 0, 1.5 and 10 min at 55 °C in a water bath. The antibiotics did not influence plant regeneration, but CLas was effectively eliminated from only 22.2% of the infected propagules of trifoliate orange up to 15 months after treatment. Thermotherapy at 55 °C for 1.5 min was ineffective in completely suppressing CLas from trifoliate orange plants eight months after treatment, and was harmful to citrus propagation, notably sweet orange. Antibiotic treatment did not eliminate CLas in sweet orange propagules, but a decrease in titer was recorded for both genotypes. MenosAbstract. Epigenetic markers related to resistance require regenerating healthy trees from previously infected plant tissues. Thus, sanitizing and regenerating healthy citrus plants from previously Candidatus Liberibacter asiaticus (CLas)-infected tissues constitute a valuable need for breeding programs. Immersion in ampicillin + streptomycin solution and thermotherapy were evaluated separately for eliminating CLas from infected citrus propagules. Valencia sweet orange (Citrus × sinensis) and trifoliate orange (Citrus trifoliata) were chosen as genotypes that were highly and moderately susceptible to CLas, respectively. Infected budwood was used to graft nucellar material with or without CLas. Thirty-two months after inoculation, 2.5-cm grafts were collected and treated by 12 h immersion in an antibiotic solution (1 g/L ampicillin + 0.1 g/L streptomycin) or in autoclaved Milli-Q water, and subsequently grafted onto Rangpur lime (C. × limonia) rootstocks. In a second experiment, thermotherapy was evaluated for treating stem cuttings kept for 0, 1.5 and 10 min at 55 °C in a water bath. The antibiotics did not influence plant regeneration, but CLas was effectively eliminated from only 22.2% of the infected propagules of trifoliate orange up to 15 months after treatment. Thermotherapy at 55 °C for 1.5 min was ineffective in completely suppressing CLas from trifoliate orange plants eight months after treatment, and was harmful to citrus propagation, notably sweet orange. Antibiot... Mostrar Tudo |
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Palavras-Chave: |
Ampicilina; Citrus trifoliata; Clonal sanitation; Huanglongbing; Saneamento clonal; Termoterapia. |
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Thesagro: |
Antibiótico. |
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Thesaurus Nal: |
Ampicillin; Candidatus Liberibacter asiaticus; Citrus; Greening disease; Streptomycin. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02749naa a2200373 a 4500
001 2169015
005 2024-11-11
008 2024 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s10658-024-02939-5$2DOI
100 1 $aMAGALHÃES, L. O.
245 $aAntibiotics and thermotherapy have limited effectiveness in eliminating Candidatus Liberibacter asiaticus from citrus budwood.$h[electronic resource]
260 $c2024
500 $aOn-line first.
520 $aAbstract. Epigenetic markers related to resistance require regenerating healthy trees from previously infected plant tissues. Thus, sanitizing and regenerating healthy citrus plants from previously Candidatus Liberibacter asiaticus (CLas)-infected tissues constitute a valuable need for breeding programs. Immersion in ampicillin + streptomycin solution and thermotherapy were evaluated separately for eliminating CLas from infected citrus propagules. Valencia sweet orange (Citrus × sinensis) and trifoliate orange (Citrus trifoliata) were chosen as genotypes that were highly and moderately susceptible to CLas, respectively. Infected budwood was used to graft nucellar material with or without CLas. Thirty-two months after inoculation, 2.5-cm grafts were collected and treated by 12 h immersion in an antibiotic solution (1 g/L ampicillin + 0.1 g/L streptomycin) or in autoclaved Milli-Q water, and subsequently grafted onto Rangpur lime (C. × limonia) rootstocks. In a second experiment, thermotherapy was evaluated for treating stem cuttings kept for 0, 1.5 and 10 min at 55 °C in a water bath. The antibiotics did not influence plant regeneration, but CLas was effectively eliminated from only 22.2% of the infected propagules of trifoliate orange up to 15 months after treatment. Thermotherapy at 55 °C for 1.5 min was ineffective in completely suppressing CLas from trifoliate orange plants eight months after treatment, and was harmful to citrus propagation, notably sweet orange. Antibiotic treatment did not eliminate CLas in sweet orange propagules, but a decrease in titer was recorded for both genotypes.
650 $aAmpicillin
650 $aCandidatus Liberibacter asiaticus
650 $aCitrus
650 $aGreening disease
650 $aStreptomycin
650 $aAntibiótico
653 $aAmpicilina
653 $aCitrus trifoliata
653 $aClonal sanitation
653 $aHuanglongbing
653 $aSaneamento clonal
653 $aTermoterapia
700 1 $aSOUZA, U.
700 1 $aRAIOL-JÚNIOR, L. L.
700 1 $aCARVALHO, E. V. de
700 1 $aMOREIRA, A. S.
700 1 $aFREITAS-ASTÚA, J. de
700 1 $aGIRARDI, E. A.
700 1 $aGESTEIRA, A. da S.
773 $tEuropean Journal of Plant Pathology, 2024.
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Registro original: |
Embrapa Agricultura Digital (CNPTIA) |
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