02749naa a2200373 a 450000100080000000500110000800800410001902400520006010000220011224501540013426000090028850000190029752016280031665000150194465000380195965000110199765000210200865000170202965000170204665300150206365300220207865300220210065300180212265300220214065300170216270000140217970000250219370000230221870000190224170000260226070000190228670000230230577300470232821690152024-11-11       2024    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.1007/s10658-024-02939-52DOI1 aMAGALHÃES, L. O.  aAntibiotics and thermotherapy have limited effectiveness in eliminating Candidatus Liberibacter asiaticus from citrus budwood.h[electronic resource]  c2024  aOn-line first.  aAbstract. Epigenetic markers related to resistance require regenerating healthy trees from previously infected plant tissues. Thus, sanitizing and regenerating healthy citrus plants from previously Candidatus Liberibacter asiaticus (CLas)-infected tissues constitute a valuable need for breeding programs. Immersion in ampicillin + streptomycin solution and thermotherapy were evaluated separately for eliminating CLas from infected citrus propagules. Valencia sweet orange (Citrus × sinensis) and trifoliate orange (Citrus trifoliata) were chosen as genotypes that were highly and moderately susceptible to CLas, respectively. Infected budwood was used to graft nucellar material with or without CLas. Thirty-two months after inoculation, 2.5-cm grafts were collected and treated by 12 h immersion in an antibiotic solution (1 g/L ampicillin + 0.1 g/L streptomycin) or in autoclaved Milli-Q water, and subsequently grafted onto Rangpur lime (C. × limonia) rootstocks. In a second experiment, thermotherapy was evaluated for treating stem cuttings kept for 0, 1.5 and 10 min at 55 °C in a water bath. The antibiotics did not influence plant regeneration, but CLas was effectively eliminated from only 22.2% of the infected propagules of trifoliate orange up to 15 months after treatment. Thermotherapy at 55 °C for 1.5 min was ineffective in completely suppressing CLas from trifoliate orange plants eight months after treatment, and was harmful to citrus propagation, notably sweet orange. Antibiotic treatment did not eliminate CLas in sweet orange propagules, but a decrease in titer was recorded for both genotypes.  aAmpicillin  aCandidatus Liberibacter asiaticus  aCitrus  aGreening disease  aStreptomycin  aAntibiótico  aAmpicilina  aCitrus trifoliata  aClonal sanitation  aHuanglongbing  aSaneamento clonal  aTermoterapia1 aSOUZA, U.1 aRAIOL-JÚNIOR, L. L.1 aCARVALHO, E. V. de1 aMOREIRA, A. S.1 aFREITAS-ASTÚA, J. de1 aGIRARDI, E. A.1 aGESTEIRA, A. da S.  tEuropean Journal of Plant Pathology, 2024.