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Biblioteca(s): |
Embrapa Instrumentação. |
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Data corrente: |
29/12/2022 |
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Data da última atualização: |
15/05/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
KATAYAMA, E.; RODRIGUES, N. A.; BILATTO, S.; CASCIATORI, F. P.; FARINAS, C. S. |
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Afiliação: |
CRISTIANE SANCHEZ FARINAS, CNPDIA. |
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Título: |
Nanocellulose isolation using a thermostable endoglucanase-rich cocktail from Myceliophthora thermophila cultivated in a multilayer packed-bed bioreactor. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Biomass Conversion and Biorefnery, v. 14, n. 8, 2022. |
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Páginas: |
9121-9136 |
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DOI: |
https://doi.org/10.1007/s13399-022-02977-1 |
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Idioma: |
Inglês |
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Conteúdo: |
The isolation of nanocellulose materials by means of enzymatic hydrolysis ofers advantages in terms of their properties and operational conditions, but there is still a lack of enzymatic cocktails specifcally designed for this application. The present work investigates the use of a thermostable endoglucanase-rich enzymatic cocktail from Myceliophthora thermophila for nanocellulose production. An initial set of experiments was conducted to determine the optimal conditions for enzyme production under solid-state cultivation in small-scale polypropylene plastic bags. A full factorial experimental design was used as a statistical tool to evaluate the efects of the solid substrate and moisture content on the production of endoglucanase and ?-glucosidase. Solid-state cultivations in a multilayer packed-bed bioreactor were then carried out under the selected conditions to obtain a larger volume of an enzymatic extract with high endoglucanase selectivity, given by the ratio of endoglucanase (203±6 U/g-substrate) to ?-glucosidase activity (1.6±0.1 U/g-substrate). The enzymatic hydrolysis of eucalyptus cellulose pulp at diferent temperatures, with this endoglucanase-rich cocktail at a loading of 10 mg-protein/g-pulp, resulted in higher glucose release (11±2 g-glucose/L) at 60 °C, with 61±9% cellulose conversion and 16.2% nanocellulose yield. The isolated nanomaterial with average diameter of 36.65 nm and length of 156.63 nm, presented a crystallinity index of 77.4% and thermal stability temperature of 316 °C. These results show the potential to isolate nanocellulose using a thermostable endoglucanase-rich enzymatic cocktail produced locally by solid-state cultivation in a packed-bed bioreactor, in accordance to the biorefnery concept. MenosThe isolation of nanocellulose materials by means of enzymatic hydrolysis ofers advantages in terms of their properties and operational conditions, but there is still a lack of enzymatic cocktails specifcally designed for this application. The present work investigates the use of a thermostable endoglucanase-rich enzymatic cocktail from Myceliophthora thermophila for nanocellulose production. An initial set of experiments was conducted to determine the optimal conditions for enzyme production under solid-state cultivation in small-scale polypropylene plastic bags. A full factorial experimental design was used as a statistical tool to evaluate the efects of the solid substrate and moisture content on the production of endoglucanase and ?-glucosidase. Solid-state cultivations in a multilayer packed-bed bioreactor were then carried out under the selected conditions to obtain a larger volume of an enzymatic extract with high endoglucanase selectivity, given by the ratio of endoglucanase (203±6 U/g-substrate) to ?-glucosidase activity (1.6±0.1 U/g-substrate). The enzymatic hydrolysis of eucalyptus cellulose pulp at diferent temperatures, with this endoglucanase-rich cocktail at a loading of 10 mg-protein/g-pulp, resulted in higher glucose release (11±2 g-glucose/L) at 60 °C, with 61±9% cellulose conversion and 16.2% nanocellulose yield. The isolated nanomaterial with average diameter of 36.65 nm and length of 156.63 nm, presented a crystallinity index of 77.4% and thermal stabili... Mostrar Tudo |
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Palavras-Chave: |
Endoglucanase; Packed-bed bioreactor; Solid-state cultivation. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02537naa a2200229 a 4500
001 2150493
005 2024-05-15
008 2022 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s13399-022-02977-1$2DOI
100 1 $aKATAYAMA, E.
245 $aNanocellulose isolation using a thermostable endoglucanase-rich cocktail from Myceliophthora thermophila cultivated in a multilayer packed-bed bioreactor.$h[electronic resource]
260 $c2022
300 $a9121-9136
520 $aThe isolation of nanocellulose materials by means of enzymatic hydrolysis ofers advantages in terms of their properties and operational conditions, but there is still a lack of enzymatic cocktails specifcally designed for this application. The present work investigates the use of a thermostable endoglucanase-rich enzymatic cocktail from Myceliophthora thermophila for nanocellulose production. An initial set of experiments was conducted to determine the optimal conditions for enzyme production under solid-state cultivation in small-scale polypropylene plastic bags. A full factorial experimental design was used as a statistical tool to evaluate the efects of the solid substrate and moisture content on the production of endoglucanase and ?-glucosidase. Solid-state cultivations in a multilayer packed-bed bioreactor were then carried out under the selected conditions to obtain a larger volume of an enzymatic extract with high endoglucanase selectivity, given by the ratio of endoglucanase (203±6 U/g-substrate) to ?-glucosidase activity (1.6±0.1 U/g-substrate). The enzymatic hydrolysis of eucalyptus cellulose pulp at diferent temperatures, with this endoglucanase-rich cocktail at a loading of 10 mg-protein/g-pulp, resulted in higher glucose release (11±2 g-glucose/L) at 60 °C, with 61±9% cellulose conversion and 16.2% nanocellulose yield. The isolated nanomaterial with average diameter of 36.65 nm and length of 156.63 nm, presented a crystallinity index of 77.4% and thermal stability temperature of 316 °C. These results show the potential to isolate nanocellulose using a thermostable endoglucanase-rich enzymatic cocktail produced locally by solid-state cultivation in a packed-bed bioreactor, in accordance to the biorefnery concept.
653 $aEndoglucanase
653 $aPacked-bed bioreactor
653 $aSolid-state cultivation
700 1 $aRODRIGUES, N. A.
700 1 $aBILATTO, S.
700 1 $aCASCIATORI, F. P.
700 1 $aFARINAS, C. S.
773 $tBiomass Conversion and Biorefnery$gv. 14, n. 8, 2022.
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Embrapa Instrumentação (CNPDIA) |
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| Registros recuperados : 75 | |
| 4. |  | CAPDEVILLE, G. de; SOUZA JUNIOR, M. T.; PAVIN, M. E. Identificação e caracterização molecular de dois genes de Beta-1,3 glucanases em Carica papaya. Fitopatologia Brasileira, v. 31, suppl., ago. 2006. Anais do: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 39.; ANNUAL MEETING OF THE BRAZILIAN PHYTOPATHOLOGICAL SOCIETY, 39., 2006, Salvador, BA. p. S327.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 8. | | CAPDEVILLE, G. de; MIRANDA, S. P.; SANTOS, J. R. Studies on the modes of action of a yeast antagonist able to control anthracnose (Colletotrichum gloesporioides) of papaya. Fitopatologia Brasileira, Brasília, DF, v. 29, supl., p. S77, ago. 2004. Edição dos Resumos do XXXVII Congresso Brasileiro de Fitopatologia, Gramado, RS, ago. 2004.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 10. | | MIRANDA, S. P. SANTOS, J. R. P.; CAPDEVILLE, G. de. Testing of yeast antogonists for the control of anthracnose (Colletorichum gloesporioides) of papaya after harvest. Fitopatologia Brasileira, Brasília, DF, v. 29, supl., p. S76, ago. 2004. Edição dos Resumos do XXXVII Congresso Brasileiro de Fitopatologia, Gramado, RS, ago. 2004.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 13. | | GOMES, A. C. M. M.; CARNEIRO, R. M. D. G.; CAPDEVILLE, G.; MATTOS, J. C. Caracterização histológica da resistência de Pfaffia glomerata a Meloidogyne incognita. Nematologia Brasileira, Brasília, v. 30, n. 1, p. 120, 2006. In: CONGRESSO BRASILEIRO DE NEMATOLOGIA, 26., 2006, Campos dos Goytacazes.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 14. | | GOMES, A. C. M. M.; CARNEIRO, R. M. D. G.; CAPDEVILLE, G.; MATTOS, J. K. A. Caracterização histológica da resistência de Pfaffia glomerata a Meloidogyne incognita. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 11., 2006, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2006. p. 47.| Tipo: Resumo em Anais de Congresso | Circulação/Nível: -- - -- |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 18. | | FALCÃO, R.; ARAUJO, A. C. G.; SOUZA JUNIOR, M. T.; CAPDEVILLE, G. Microsporogêneses em banana triploide AAA (Musa acuminata). In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 13., 2008, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2008. Resumo 014. p. 52.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 19. | | FALCÃO, R.; SOUZA JÚNIOR, M. T.; ARAUJO, A. C. G.; CAPDEVILLE, G. Microsporogenesis in triploid banana (Musa spp.). In: SIMPÓSIO DE MICROSCOPIA NO CERRADO, 2008, Pirenópolis, GO. [Anais...] Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2008. 321 p. (Embrapa Recursos Genéticos e Biotecnologia, 265) Organizadoras: Ana Claudia Guerra de Araújo e Sônia Nair Báo. p. 266-269.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| Registros recuperados : 75 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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