Registro Completo |
Biblioteca(s): |
Embrapa Gado de Leite. |
Data corrente: |
25/11/2021 |
Data da última atualização: |
25/11/2021 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
OLIVEIRA, C. S.; MARQUES, S. C. S.; GUEDES, P. H. E.; FEUCHARD, V. L.; CAMARGO, A. J. R.; FREITAS, C. de; CAMARGO, L. S. de A. |
Afiliação: |
CLARA SLADE OLIVEIRA, CNPGL; SHEILA C. S. MARQUES; PEDRO H. E. GUEDES; VIVIANE L. FEUCHARD, Pesagro; AGOSTINHO J. R. CAMARGO, Pesagro; CELIO DE FREITAS, CNPGL; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL. |
Título: |
Thermal-treatment protocol to induce thermotolerance in bovine embryos. |
Ano de publicação: |
2021 |
Fonte/Imprenta: |
Reproduction, Fertility and Development, v. 33, n. 7, p. 497-501, 2021. |
DOI: |
https://doi.org/10.1071/RD20309 |
Idioma: |
Inglês |
Conteúdo: |
Artificial reproduction in dairy cattle is challenged by summer temperatures in tropical environments. We describe a treatment based on mild temperature increasesto induce thermotolerance and improve the embryo?s performance under heat stress conditions. A protocol was established to induce upregulation of heat shock protein A (HSPA, formerly known as HSP70) but not impair embryonic development. Thermal treatment(TT) had no effect onmorula/blastocyst rate or blastocyst quality (cell number and apoptosis). Heatshock given one day after TTrevealed higher(P ¼ 0.00)survivalratesin TT blastocysts compared with Control. Treated embryos were transferred to recipients and no detrimental effects were observed regarding pregnancy rates, length, fetal growth or calf weight. Our results demonstrated that the established TT protocol could induce a thermal response by the embryo and is safe for further development. |
Palavras-Chave: |
Choque térmico; Embryo survival; Estresse térmico; HSP; IVF; IVP; Sobrevivência embrionária. |
Thesagro: |
Bovino. |
Thesaurus Nal: |
Heat shock response; Thermal stress. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
Marc: |
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Registro original: |
Embrapa Gado de Leite (CNPGL) |