01852naa a2200325 a 450000100080000000500110000800800410001902400410006010000200010124500990012126000090022052009200022965000240114965000190117365000110119265300200120365300200122365300220124365300080126565300080127365300080128165300320128970000220132170000210134370000200136470000220138470000190140670000250142577300760145021365462021-11-25       2021    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.1071/RD203092DOI1 aOLIVEIRA, C. S.  aThermal-treatment protocol to induce thermotolerance in bovine embryos.h[electronic resource]  c2021  aArtificial reproduction in dairy cattle is challenged by summer temperatures in tropical environments. We describe a treatment based on mild temperature increasesto induce thermotolerance and improve the embryo?s performance under heat stress conditions. A protocol was established to induce upregulation of heat shock protein A (HSPA, formerly known as HSP70) but not impair embryonic development. Thermal treatment(TT) had no effect onmorula/blastocyst rate or blastocyst quality (cell number and apoptosis). Heatshock given one day after TTrevealed higher(P ¼ 0.00)survivalratesin TT blastocysts compared with Control. Treated embryos were transferred to recipients and no detrimental effects were observed regarding pregnancy rates, length, fetal growth or calf weight. Our results demonstrated that the established TT protocol could induce a thermal response by the embryo and is safe for further development.  aHeat shock response  aThermal stress  aBovino  aChoque térmico  aEmbryo survival  aEstresse térmico  aHSP  aIVF  aIVP  aSobrevivência embrionária1 aMARQUES, S. C. S.1 aGUEDES, P. H. E.1 aFEUCHARD, V. L.1 aCAMARGO, A. J. R.1 aFREITAS, C. de1 aCAMARGO, L. S. de A.  tReproduction, Fertility and Developmentgv. 33, n. 7, p. 497-501, 2021.