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Biblioteca(s): |
Embrapa Arroz e Feijão; Embrapa Meio Ambiente. |
Data corrente: |
06/05/2020 |
Data da última atualização: |
06/05/2020 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
LOPES, A. R. de O.; LOCATELLI, G. O.; BARBOSA, R. de M.; LOBO JUNIOR, M.; MASCARIN, G. M.; FINKLER, C. L. L. |
Afiliação: |
ALLYSON RODRIGO DE OLIVEIRA LOPES, UNIVERSIDADE FEDERAL DE PERNAMBUCO; GABRIEL OLIVO LOCATELLI, UNIVERSIDADE FEDERAL DE PERNAMBUCO; RAQUEL DE MELO BARBOSA, UNINASSAU; MURILLO LOBO JUNIOR, CNPAF; GABRIEL MOURA MASCARIN, CNPMA; CHRISTINE LAMENHA LUNA FINKLER, UNIVERSIDADE FEDERAL DE PERNAMBUCO. |
Título: |
Preparation, characterisation and cell viability of encapsulated Trichoderma asperellumin alginate beads. |
Ano de publicação: |
2020 |
Fonte/Imprenta: |
Journal of Microencapsulation, v. 37, n. 3, p. 270-282, 2020. |
ISSN: |
0265-2048 |
DOI: |
https://doi.org/10.1080/02652048.2020.1729884 |
Idioma: |
Inglês |
Conteúdo: |
Aim: The encapsulation of Trichoderma asperellum BRM-29104 using Ca-alginate matrix was evaluated. Methods: Intact and freeze-dried beads containing submerged conidia and microsclerotia (MS) of T. asperellum grown in liquid culture were prepared and characterised. Beads were stored at 8, 25, and 35 oC over 120 days. Results: The mean sizes of beads before and after freeze-drying were 2.5 ± 0.2mm and 1.5 x 1.1mm (± 0.1mm), respectively. Freeze-dried beads stored at 8 oC were more effective in maintaining conidia concentration, while MS concentrations yielded 102 MS/g for both beads at 8 and 25 oC. The concentration of viable cells in freeze-dried beads stored at 8 oC attained 3.0 x 108 CFU/g after 120 days. FIRT analysis showed an interaction between the alginate and the cell wall of the fungus. Conclusion: These findings support the use of alginate beads followed by freeze drying and cold storage for maintenance of viability of T. asperellum. |
Palavras-Chave: |
Biocontrol; Microsclerotia. |
Thesagro: |
Fungo; Trichoderma. |
Thesaurus Nal: |
Alginates; Encapsulation; Fungi; Trichoderma asperellum. |
Categoria do assunto: |
H Saúde e Patologia |
Marc: |
LEADER 01859naa a2200301 a 4500 001 2122103 005 2020-05-06 008 2020 bl uuuu u00u1 u #d 022 $a0265-2048 024 7 $ahttps://doi.org/10.1080/02652048.2020.1729884$2DOI 100 1 $aLOPES, A. R. de O. 245 $aPreparation, characterisation and cell viability of encapsulated Trichoderma asperellumin alginate beads.$h[electronic resource] 260 $c2020 520 $aAim: The encapsulation of Trichoderma asperellum BRM-29104 using Ca-alginate matrix was evaluated. Methods: Intact and freeze-dried beads containing submerged conidia and microsclerotia (MS) of T. asperellum grown in liquid culture were prepared and characterised. Beads were stored at 8, 25, and 35 oC over 120 days. Results: The mean sizes of beads before and after freeze-drying were 2.5 ± 0.2mm and 1.5 x 1.1mm (± 0.1mm), respectively. Freeze-dried beads stored at 8 oC were more effective in maintaining conidia concentration, while MS concentrations yielded 102 MS/g for both beads at 8 and 25 oC. The concentration of viable cells in freeze-dried beads stored at 8 oC attained 3.0 x 108 CFU/g after 120 days. FIRT analysis showed an interaction between the alginate and the cell wall of the fungus. Conclusion: These findings support the use of alginate beads followed by freeze drying and cold storage for maintenance of viability of T. asperellum. 650 $aAlginates 650 $aEncapsulation 650 $aFungi 650 $aTrichoderma asperellum 650 $aFungo 650 $aTrichoderma 653 $aBiocontrol 653 $aMicrosclerotia 700 1 $aLOCATELLI, G. O. 700 1 $aBARBOSA, R. de M. 700 1 $aLOBO JUNIOR, M. 700 1 $aMASCARIN, G. M. 700 1 $aFINKLER, C. L. L. 773 $tJournal of Microencapsulation$gv. 37, n. 3, p. 270-282, 2020.
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1. |  | VALERIO, J. R.; TORRES, F. Z. V.; MORAES, L. R. O; KALACHE, S. R.; STEFANELLO, A. M.; OLIVEIRA, M. C. M. Nível de tolerância em genótipos promissores do gênero brachiaria quanto aos danos causados por adultos da cigarrinha-das-pastagens deois Flavopicta (Hemiptera: Cercopidae). O Biológico, São Paulo, SP, v. 75, n. 2, p 93 , jul./dez. 2013. Edição dos resumos da 26ª Reunião Anual do Instituto Biológico, São Paulo, 2013 e 11º Congresso de Iniciação Científica em Ciências Agrárias Biológicas e Ambientais - CICAM, agosto, 2013. Resumo 064.Tipo: Resumo em Anais de Congresso |
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