01859naa a2200301 a 450000100080000000500110000800800410001902200140006002400550007410000230012924501330015226000090028552009630029465000140125765000180127165000100128965000270129965000100132665000160133665300150135265300190136770000210138670000220140770000200142970000200144970000220146977300660149121221032020-05-06       2020    bl uuuu     u00u1 u    #d  a0265-20487 ahttps://doi.org/10.1080/02652048.2020.17298842DOI1 aLOPES, A. R. de O.  aPreparation, characterisation and cell viability of encapsulated Trichoderma asperellumin alginate beads.h[electronic resource]  c2020  aAim: The encapsulation of Trichoderma asperellum BRM-29104 using Ca-alginate matrix was evaluated. Methods: Intact and freeze-dried beads containing submerged conidia and microsclerotia (MS) of T. asperellum grown in liquid culture were prepared and characterised. Beads were stored at 8, 25, and 35 oC over 120 days. Results: The mean sizes of beads before and after freeze-drying were 2.5 ± 0.2mm and 1.5 x 1.1mm (± 0.1mm), respectively. Freeze-dried beads stored at 8 oC were more effective in maintaining conidia concentration, while MS concentrations yielded 102 MS/g for both beads at 8 and 25 oC. The concentration of viable cells in freeze-dried beads stored at 8 oC attained 3.0 x 108 CFU/g after 120 days. FIRT analysis showed an interaction between the alginate and the cell wall of the fungus. Conclusion: These findings support the use of alginate beads followed by freeze drying and cold storage for maintenance of viability of T. asperellum.  aAlginates  aEncapsulation  aFungi  aTrichoderma asperellum  aFungo  aTrichoderma  aBiocontrol  aMicrosclerotia1 aLOCATELLI, G. O.1 aBARBOSA, R. de M.1 aLOBO JUNIOR, M.1 aMASCARIN, G. M.1 aFINKLER, C. L. L.  tJournal of Microencapsulationgv. 37, n. 3, p. 270-282, 2020.