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 | Acesso ao texto completo restrito à biblioteca da Embrapa Meio Ambiente. Para informações adicionais entre em contato com cnpma.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
09/12/2019 |
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Data da última atualização: |
10/06/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MARTINEZ, A. F. C.; MELLO, F. M. P.; ZUCCHI, T. D.; MELO, I. S. de; MORAES, L. A. B. |
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Afiliação: |
ANA FLÁVIA CANOVAS MARTINEZ, FFCLRP-USP; FLÁVIA MADOLESI PEREIRA MELLO; TIAGO DOMINGUES ZUCCHI; ITAMAR SOARES DE MELO, CNPMA; LUIZ ALBERTO BERALDO MORAES, FFCLRP-USP. |
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Título: |
Tandem mass spectrometry methods to accelerate the identification of phytotoxic metabolites produced by Streptomyces sp. 39 PL. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Natural Product Research, v. 34, n. 2, p. 210-216, 2019. |
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DOI: |
https://doi.org/10.1080/14786419.2018.1525713 |
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Idioma: |
Inglês |
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Notas: |
Arquivo com texto completo contém a versão publicada online, sem dados de volume e paginação. |
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Conteúdo: |
Abstract: Natural products isolated by microorganisms are interesting in the search for new compounds with several biological activities. However, low concentration and structural diversity make the isolation a time-consuming step. Tandem mass spectrometry is a well-established technology for the identification and characterization of target microbial natural products due to high sensitivity and selectivity of these experiments. We developed a method employing neutral loss experiments (LC-ESI-MS/MS) to identify luminacins in microbial crude extracts. The luminacins class exhibited conserved fragmentation pattern with loss at 172Da relative to glycosides fragment and this loss was used in searching for compounds belonging to this class. Therefore, the crude extract produced by Streptomyces sp. 39 PL was analysed and five luminacins were isolated - one is a novel luminacin I at 466Da. |
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Palavras-Chave: |
Dereplication; Luminacins; Neutral loss experiments. |
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Thesagro: |
Análise Química; Bactéria; Identificação; Método de Análise. |
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Thesaurus Nal: |
phytotoxins; Species identification; Streptomyces; Tandem mass spectrometry. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
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001 2116318
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008 2019 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1080/14786419.2018.1525713$2DOI
100 1 $aMARTINEZ, A. F. C.
245 $aTandem mass spectrometry methods to accelerate the identification of phytotoxic metabolites produced by Streptomyces sp. 39 PL.$h[electronic resource]
260 $c2019
500 $aArquivo com texto completo contém a versão publicada online, sem dados de volume e paginação.
520 $aAbstract: Natural products isolated by microorganisms are interesting in the search for new compounds with several biological activities. However, low concentration and structural diversity make the isolation a time-consuming step. Tandem mass spectrometry is a well-established technology for the identification and characterization of target microbial natural products due to high sensitivity and selectivity of these experiments. We developed a method employing neutral loss experiments (LC-ESI-MS/MS) to identify luminacins in microbial crude extracts. The luminacins class exhibited conserved fragmentation pattern with loss at 172Da relative to glycosides fragment and this loss was used in searching for compounds belonging to this class. Therefore, the crude extract produced by Streptomyces sp. 39 PL was analysed and five luminacins were isolated - one is a novel luminacin I at 466Da.
650 $aphytotoxins
650 $aSpecies identification
650 $aStreptomyces
650 $aTandem mass spectrometry
650 $aAnálise Química
650 $aBactéria
650 $aIdentificação
650 $aMétodo de Análise
653 $aDereplication
653 $aLuminacins
653 $aNeutral loss experiments
700 1 $aMELLO, F. M. P.
700 1 $aZUCCHI, T. D.
700 1 $aMELO, I. S. de
700 1 $aMORAES, L. A. B.
773 $tNatural Product Research$gv. 34, n. 2, p. 210-216, 2019.
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| 1. |  | SCHLAGHAMERSKY, J.; SIDOVÁ, A. Dynamics and vertical distribution of a Hrabeiella periglandulata (Annelida) population in South Moravia, Czech Republic. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 44, n. 8, p. 917-921, ago. 2009 Título em português: Dinâmica e distribuição vertical de uma população de Hrabeiella periglandulata (Annelida) no Sul da Morávia, República Tcheca.| Biblioteca(s): Embrapa Unidades Centrais. |
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