02000naa a2200325 a 450000100080000000500110000800800410001902400550006010000230011524501550013826000090029350001020030252009000040465000160130465000270132065000170134765000290136465000220139365000140141565000200142965000240144965300180147365300150149165300290150670000200153570000180155570000190157370000210159277300610161321163182022-06-10       2019    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.1080/14786419.2018.15257132DOI1 aMARTINEZ, A. F. C.  aTandem mass spectrometry methods to accelerate the identification of phytotoxic metabolites produced by Streptomyces sp. 39 PL.h[electronic resource]  c2019  aArquivo com texto completo contém a versão publicada online, sem dados de volume e paginação.  aAbstract: Natural products isolated by microorganisms are interesting in the search for new compounds with several biological activities. However, low concentration and structural diversity make the isolation a time-consuming step. Tandem mass spectrometry is a well-established technology for the identification and characterization of target microbial natural products due to high sensitivity and selectivity of these experiments. We developed a method employing neutral loss experiments (LC-ESI-MS/MS) to identify luminacins in microbial crude extracts. The luminacins class exhibited conserved fragmentation pattern with loss at 172Da relative to glycosides fragment and this loss was used in searching for compounds belonging to this class. Therefore, the crude extract produced by Streptomyces sp. 39 PL was analysed and five luminacins were isolated - one is a novel luminacin I at 466Da.  aphytotoxins  aSpecies identification  aStreptomyces  aTandem mass spectrometry  aAnálise Química  aBactéria  aIdentificação  aMétodo de Análise  aDereplication  aLuminacins  aNeutral loss experiments1 aMELLO, F. M. P.1 aZUCCHI, T. D.1 aMELO, I. S. de1 aMORAES, L. A. B.  tNatural Product Researchgv. 34, n. 2, p. 210-216, 2019.