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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
10/07/2018 |
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Data da última atualização: |
18/09/2018 |
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Tipo da produção científica: |
Capítulo em Livro Técnico-Científico |
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Autoria: |
SOUZA, F. V. D.; SOUZA, E. H. de; SILVA, R. L. da. |
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Afiliação: |
FERNANDA VIDIGAL DUARTE SOUZA, CNPMF; EVERTON HILO DE SOUZA; RONILZE LEITE DA SILVA. |
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Título: |
Cryopreservation of pollen grains of pineapple and other bromeliads. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
In: LOYOLA-VARGAS, V.r M; Ochoa-Alejo, N. (ed.). Plant Cell Culture Protocols, Methods in Molecular Biology. New York, NY: Humana Press, 2018. |
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Páginas: |
279-288 |
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Descrição Física: |
v.1815. Cap. 19. |
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DOI: |
0.1007/978-1-4939-8594-4_19 |
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Idioma: |
Inglês |
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Conteúdo: |
Cryopreservation of pollen grains is an efficient technique to overcome asynchronous flowering and to support actions for genetic improvement and conservation of important alleles. It can be used both by germplasm curators and plant breeders. In the case of Bromeliaceae, a family with wide diversity but also high vulnerability, the form of conservation can be crucial to prevent the increasing problem of genetic erosion. This chapter describes a method of cryopreservation of pollen grains of different Bromeliaceae species, including pineapple, after dehydration with silica and subsequent immersion in liquid nitrogen. The efficiency of this protocol has been demonstrated by the high pollen viability percentage and produc-tion of seeds after in vivo pollination with cryopreserved grains. The protocol can be used for cryopreserv-ing pollen of many species of bromeliads and is easy to perform. |
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Thesagro: |
Bromeliaceae; Conservação; Criopreservação; Melhoramento Vegetal. |
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Thesaurus Nal: |
Breeding and Genetic Improvement; Conservation plants; Cryopreservation. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01763naa a2200253 a 4500
001 2093161
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008 2018 bl uuuu u00u1 u #d
024 7 $a0.1007/978-1-4939-8594-4_19$2DOI
100 1 $aSOUZA, F. V. D.
245 $aCryopreservation of pollen grains of pineapple and other bromeliads.$h[electronic resource]
260 $c2018
300 $a279-288$cv.1815. Cap. 19.
520 $aCryopreservation of pollen grains is an efficient technique to overcome asynchronous flowering and to support actions for genetic improvement and conservation of important alleles. It can be used both by germplasm curators and plant breeders. In the case of Bromeliaceae, a family with wide diversity but also high vulnerability, the form of conservation can be crucial to prevent the increasing problem of genetic erosion. This chapter describes a method of cryopreservation of pollen grains of different Bromeliaceae species, including pineapple, after dehydration with silica and subsequent immersion in liquid nitrogen. The efficiency of this protocol has been demonstrated by the high pollen viability percentage and produc-tion of seeds after in vivo pollination with cryopreserved grains. The protocol can be used for cryopreserv-ing pollen of many species of bromeliads and is easy to perform.
650 $aBreeding and Genetic Improvement
650 $aConservation plants
650 $aCryopreservation
650 $aBromeliaceae
650 $aConservação
650 $aCriopreservação
650 $aMelhoramento Vegetal
700 1 $aSOUZA, E. H. de
700 1 $aSILVA, R. L. da
773 $tIn: LOYOLA-VARGAS, V.r M; Ochoa-Alejo, N. (ed.). Plant Cell Culture Protocols, Methods in Molecular Biology. New York, NY: Humana Press, 2018.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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| 1. |  | COSENDEY-KEZENLEITE, R. I. J.; OLIVEIRA, F. C. R. de; FRAZÃO-TEIXEIRA, E.; DUBEY, J. P.; SOUZA, G. N. de; FERREIRA, A. M. R.; LILENBAUM, W. Occurrence and risk factors associated to Toxoplasma gondii infection in sheep from Rio de Janeiro, Brazil. Tropical Animal Health and Production, v. 46, n. 8, p. 1463-1466, 2014.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 2. | | ANDRADE, L. H. M.; LUGARINI, C.; OLIVEIRA, R. A. S.; SILVA, L. T. R.; MARVULO, M. F. V.; GARCIA, J. E.; DUBEY, J. P.; SILVA, J. C. R. Ocorrência de anticorpos anti-Toxoplasma gondii em aves silvestres de três Unidades de Conservação Federais da Paraíba e da Bahia. Pesquisa Veterinária Brasileira,Rio de Janeiro, v. 36, n. 2, p. 103-107, fev. 2016.| Biblioteca(s): Embrapa Unidades Centrais. |
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